Han Kaiou, Mao Xinyu, Liu Hui, Wu Yuxing, Tan Yizhou, Li Ziyong, Ma Runwen, Li Yinan, Li Lei, Wang Leping, Shi Yan, Cao Yajie, Peng Hao, Li Xun, Wang Xiaoye
College of Animal Science and Technology, Guangxi University, Nanning, 530004, Guangxi, People's Republic of China.
Guangxi Zhuang Autonomous Region Engineering Research Center of Veterinary Biologics, Nanning, 530004, Guangxi, China.
Virus Genes. 2023 Apr;59(2):302-311. doi: 10.1007/s11262-023-01966-4. Epub 2023 Jan 26.
Escherichia coli is a common conditional pathogen, for which antibiotic therapy is considered an effective treatment. The imprudent use of antibiotics has led to the increase of multiple-antibiotic-resistant E. coli species. With the incidence of antibiotic resistance reaching a crisis point, it is imperative to find alternative treatments for multidrug-resistant infections. Using phage for pathogen control is a promising treatment option to combat bacterial resistance. In this study, a novel virulent Podoviridae phage Kayfunavirus TM1 infecting Escherichia coli was isolated from pig farm sewage in Guangxi, China. The one-step growth curve with the optimal multiplicity of infection of 0.01 revealed a latent period of 10 min and a burst size of 50 plaque-forming units per cell. The stability test reveals that it is stable from 4 to 60 °C and pH from 3 to 11. The double-stranded DNA genome of phage Kayfunavirus TM1 is composed of 39,948 base pairs with a GC content of 50.03%.
大肠杆菌是一种常见的条件致病菌，抗生素治疗被认为是针对它的有效治疗方法。抗生素的不当使用导致了多重耐药大肠杆菌菌株的增加。随着抗生素耐药性的发生率达到危机点，寻找针对多重耐药感染的替代治疗方法势在必行。使用噬菌体控制病原体是对抗细菌耐药性的一种有前景的治疗选择。在本研究中，从中国广西的猪场污水中分离出一种新型的、能感染大肠杆菌的烈性短尾噬菌体科凯富纳病毒TM1。一步生长曲线显示，最佳感染复数为0.01时，潜伏期为10分钟，每个细胞的裂解量为50个噬菌斑形成单位。稳定性测试表明，它在4至60°C以及pH值为3至11的条件下都很稳定。噬菌体凯富纳病毒TM1的双链DNA基因组由39948个碱基对组成，GC含量为50.03%。
