Inotiv, P.O. Box 13501, Research Triangle Park, NC, 27709, USA.
U.S. Environmental Protection Agency, Office of Pesticide Programs, MC7507M, 1200 Pennsylvania Ave. NW, Washington, DC, 20460, USA.
Regul Toxicol Pharmacol. 2023 Mar;139:105340. doi: 10.1016/j.yrtph.2023.105340. Epub 2023 Jan 23.
The U.S. Environmental Protection Agency (USEPA) uses the in vivo fish acute toxicity test to assess potential risk of substances to non-target aquatic vertebrates. The test is typically conducted on a cold and a warm freshwater species and a saltwater species for a conventional pesticide registration, potentially requiring upwards of 200 or more fish. A retrospective data evaluation was conducted to explore the potential for using fewer fish species to support conventional pesticide risk assessments. Lethal concentration 50% (LC) values and experimental details were extracted and curated from 718 studies on fish acute toxicity submitted to USEPA. The LC data were analysed to determine, when possible, the relative sensitivity of the tested species to each pesticide. One of the tested freshwater species was most sensitive in 85% of those cases. The tested cold freshwater species was the most sensitive overall among cases with established relative sensitivity and was within 3X of the LC value of the most sensitive species tested in 98% of those cases. The results support potentially using fewer than three fish species to conduct ecological risk assessments for the registration of conventional pesticides.
美国环境保护署（USEPA）使用体内鱼类急性毒性测试来评估物质对非目标水生脊椎动物的潜在风险。通常在冷水和温水淡水物种以及海水物种上进行常规农药登记测试，可能需要多达 200 多条或更多的鱼。进行了回顾性数据分析，以探讨使用较少的鱼类物种来支持常规农药风险评估的可能性。从提交给 USEPA 的 718 项鱼类急性毒性研究中提取并整理了致死浓度 50%（LC）值和实验细节。对 LC 数据进行了分析，以确定测试物种对每种农药的相对敏感性（如果可能的话）。在所研究的 85%的情况下，一种测试淡水物种最敏感。在所研究的具有确定相对敏感性的情况下，冷淡水物种总体上最敏感，在 98%的情况下，其 LC 值与测试的最敏感物种的 LC 值相差 3 倍以内。结果支持使用少于三种鱼类物种来进行常规农药登记的生态风险评估。
