State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China.
State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China.
Int J Biol Macromol. 2023 Mar 1;230:123401. doi: 10.1016/j.ijbiomac.2023.123401. Epub 2023 Jan 24.
Histones are essential components of chromatin and play an important role in regulating gene transcription and participating in DNA replication. Here, we performed a comprehensive analysis of this gene family. In this study, we identified 37 CsHistones that were classified into five groups (H1, H2A, H2B, H3 and H4). The closely linked subfamilies exhibited more similarity in terms of motifs and intron/exon numbers. Segmental duplication (SD) is the main driving force of cucumber CsHistones expansion. Analysis of cis-regulatory elements in the promoter region of CsHistones showed that CsHistones can respond to a variety of stresses. RNA-Seq analysis indicated that the expression of most CsHistones was associated with different stresses, including downy mildew, powdery mildew, wilt, heat, cold, salt stress, and waterlogging. Expression analysis showed that several genes of H3 group were highly expressed in different reproductive organs. Notably, CsCENH3 (CsHistone30) has the characteristics of a variant histone, and we demonstrated that CsCENH3 was localized on the nucleus and its proteins were expressed in centromere region. These findings provide valuable information for the identification and potential functions of Histone genes and ideas for the cultivation of CENH3-mediated haploid induction lines in cucumber.
组蛋白是染色质的重要组成部分，在调节基因转录和参与 DNA 复制中发挥着重要作用。在这里，我们对这个基因家族进行了全面的分析。在这项研究中，我们鉴定了 37 个 CsHistones，它们被分为五个亚家族（H1、H2A、H2B、H3 和 H4）。紧密连锁的亚家族在基序和内含子/外显子数量方面表现出更高的相似性。片段复制（SD）是黄瓜 CsHistones 扩张的主要驱动力。对 CsHistones 启动子区顺式调控元件的分析表明，CsHistones 可以对多种胁迫做出响应。RNA-Seq 分析表明，大多数 CsHistones 的表达与不同的胁迫有关，包括霜霉病、白粉病、枯萎病、热、冷、盐胁迫和涝渍。表达分析表明，H3 组的几个基因在不同的生殖器官中高表达。值得注意的是，CsCENH3（CsHistone30）具有变体组蛋白的特征，我们证明 CsCENH3 定位于细胞核，其蛋白在着丝粒区域表达。这些发现为鉴定组蛋白基因的潜在功能提供了有价值的信息，并为培育黄瓜中 CENH3 介导的单倍体诱导系提供了思路。
