Asadi Azita, Goudarzi Farjam, Ghanadian Mustafa, Mohammadalipour Adel
Department of Clinical Biochemistry and Isfahan Pharmaceutical Sciences Research Center, Isfahan University of Medical Sciences, Isfahan, I.R. Iran.
Regenerative Medicine Research Center, Kermanshah University of Medical Sciences, Kermanshah, I.R. Iran.
Res Pharm Sci. 2022 Oct 29;17(6):697-706. doi: 10.4103/1735-5362.359436. eCollection 2022 Dec.
Apigenin has stimulatory effects on osteogenic differentiation of human mesenchymal stem cells (hMSCs) as well as anti-inflammatory properties. This study investigated the osteogenic differentiation of hMSCs in inflammatory conditions treated with apigenin focusing on nuclear factor kappa-light-chain-enhancer of activated B (NF-кB), nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha (IκBα) and nucleotide-binding oligomerization domain, leucine-rich repeat and pyrin domain containing 3 (NLRP3) inflammatory pathways.
Along with osteogenic differentiation of the hMSCs, they became inflamed with lipopolysaccharide (LPS)/palmitic acid (PA) and treated with apigenin. Alizarin red staining, alkaline phosphatase (ALP) activity, and Runt-related transcription factor 2 (RUNX2) gene expression were used to determine the degree of differentiation. Also, gene expression of NLRP3 was performed along with protein expression of interleukin 1-beta (IL-1β), NF-кB, and IκBα.
FINDINGS / RESULTS: Apigenin was shown to be effective in neutralizing the inhibitory impact of LPS/PA on osteogenesis. Apigenin increased MSC osteogenic capacity by inhibiting NLRP3 expression and the activity of caspase-1. It was also associated with a considerable decrease in the protein expression of NF-κB and IκBα, as well as IL-1β, in these cells.
The effects of apigenin on osteogenesis under inflammatory conditions were cautiously observed.
芹菜素对人间充质干细胞（hMSCs）的成骨分化具有刺激作用，同时还具有抗炎特性。本研究聚焦于活化B细胞核因子κ轻链增强子（NF-кB）、B细胞中κ轻链多肽基因增强子的核因子抑制剂α（IκBα）和含核苷酸结合寡聚化结构域、富含亮氨酸重复序列和pyrin结构域3（NLRP3）炎症途径，研究了芹菜素处理炎症条件下hMSCs的成骨分化。
随着hMSCs的成骨分化，它们用脂多糖（LPS）/棕榈酸（PA）诱导炎症，并使用芹菜素处理。茜素红染色、碱性磷酸酶（ALP）活性和 runt 相关转录因子 2（RUNX2）基因表达用于确定分化程度。此外，还进行了 NLRP3 的基因表达以及白细胞介素 1β（IL-1β）、NF-кB 和 IκBα 的蛋白表达检测。
结果表明，芹菜素可有效中和 LPS/PA 对成骨的抑制作用。芹菜素通过抑制 NLRP3 表达和半胱天冬酶 -1 的活性来提高 MSC 的成骨能力。这也与这些细胞中 NF-κB、IκBα 以及 IL-1β 的蛋白表达显著降低有关。
谨慎观察了芹菜素在炎症条件下对成骨的影响。
