Yusefi Mostafa, Shameli Kamyar, Lee-Kiun Michiele Soon, Teow Sin-Yeang, Moeini Hassan, Ali Roshafima Rasit, Kia Pooneh, Jie Chia Jing, Abdullah Nurul Hidayah
Malaysia-Japan International Institute of Technology, Universiti Teknologi Malaysia, Jalan Sultan Yahya Petra, 54100 Kuala Lumpur, Malaysia; Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia.
Malaysia-Japan International Institute of Technology, Universiti Teknologi Malaysia, Jalan Sultan Yahya Petra, 54100 Kuala Lumpur, Malaysia; Institute of Virology, School of Medicine, Technical University of Munich, 81675 Munich, Germany.
Int J Biol Macromol. 2023 Apr 1;233:123388. doi: 10.1016/j.ijbiomac.2023.123388. Epub 2023 Jan 24.
Polysaccharide-based magnetic nanocomposites can eminently illuminate several attractive features as anticancer drug carriers. In this study, rice straw-based cellulose nanowhisker (CNW) was used as solid support for FeO nanofillers to synthesize magnetic CNW. Then, cross-linked chitosan-coated magnetic CNW for 5-fluorouracil carrier abbreviated as CH/MCNW/5FU. Fourier-transform infrared, X-Ray diffraction, and X-ray photoelectron spectroscopy analysis indicated successful fabrication and multifunctional properties of the CH/MCNW/5FU nanocomposites. In addition, CH/MCNW/5FU nanocomposites showed hydrodynamic diameter and zeta potential value of 181.31 ± 3.46 nm and +23 ± 1.8 mV, respectively. Based on images of transmission electron microscopy, magnetic CNW as reinforcement was coated with chitosan to obtain almost spherical CH/MCNW/5FU nanocomposites with an average diameter of 37.16 ± 3.08. The nanocomposites indicated desired saturation magnetization and thermal stability, high drug encapsulation efficiency, and pH-dependent swelling and drug release performance. CH/MCNW/5FU nanocomposites showed potent killing effects against colorectal cancer cells in both 2D monolayer and 3D spheroid models. These findings suggest CH/MCNW as a potential carrier for anticancer drugs with high tumour-penetrating capacity.
基于多糖的磁性纳米复合材料作为抗癌药物载体具有诸多吸引人的特性。在本研究中，以稻草为原料的纤维素纳米晶须（CNW）被用作FeO纳米填料的固体载体，以合成磁性CNW。然后，制备了用于5-氟尿嘧啶载体的交联壳聚糖包覆磁性CNW，简称为CH/MCNW/5FU。傅里叶变换红外光谱、X射线衍射和X射线光电子能谱分析表明CH/MCNW/5FU纳米复合材料制备成功且具有多功能特性。此外，CH/MCNW/5FU纳米复合材料的流体动力学直径和zeta电位值分别为181.31±3.46 nm和+23±1.8 mV。基于透射电子显微镜图像，作为增强材料的磁性CNW被壳聚糖包覆，从而获得平均直径为37.16±3.08的近乎球形的CH/MCNW/5FU纳米复合材料。该纳米复合材料具有所需的饱和磁化强度和热稳定性、高药物包封效率以及pH依赖性溶胀和药物释放性能。CH/MCNW/5FU纳米复合材料在二维单层和三维球体模型中均对结肠癌细胞显示出强大的杀伤作用。这些发现表明CH/MCNW作为一种具有高肿瘤穿透能力的抗癌药物潜在载体。
