Faculty of Pharmaceutical Sciences, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran.
Department of Chemistry, College of Basic Sciences, Central Tehran Branch, Islamic Azad University, Tehran, Iran.
Int J Biol Macromol. 2023 Mar 1;230:123380. doi: 10.1016/j.ijbiomac.2023.123380. Epub 2023 Jan 25.
Liposomes and nanofibers have been introduced as effective drug delivery systems of anticancer drugs. The performance of chitosan (core)/poly(ε-caprolactone) (PCL)/paclitaxel simple nanofibers, chitosan/paclitaxel (core)/PCL/chitosan (shell) nanofibers and paclitaxel-loaded liposome-incorporated chitosan (core)/PCL-chitosan (shell) nanofibers was investigated for the controlled release of paclitaxel and the treatment of breast cancer. The synthesized formulations were characterized using polydispersity index, dynamic light scattering, zeta potential, scanning electron microscopy, transmission electron microscopy, and Fourier transform infrared analysis. The sustained release of paclitaxel from liposome-loaded nanofibers was achieved within 30 days. The release data was best described using Korsmeyer-Peppas pharmacokinetic model. The cell viabilities of synthesized nanofibrous samples were higher than 98 % ± 1 % toward L929 normal cells after 168 h. The maximum cytotoxicity against MCF-7 breast cancer cells was 85 % ± 2.5 % using liposome-loaded core-shell nanofibers. The in vivo results indicated the reduction of tumor weight from 1.35 ± 0.15 g to 0.65 ± 0.05 g using liposome-loaded core-shell nanofibers and its increasing from 1.35 ± 0.15 g to 3.2 ± 0.2 g using pure core-shell nanofibers. The three-stage drug release behavior of paclitaxel-loaded liposome-incorporated core-shell nanofibers and the high in vivo tumor efficiency suggested the development of these formulations for cancer treatment in the future.
脂质体和纳米纤维已被引入作为抗癌药物的有效药物递送系统。壳聚糖（核）/聚（ε-己内酯）（PCL）/紫杉醇简单纳米纤维、壳聚糖/紫杉醇（核）/PCL/壳聚糖（壳）纳米纤维和载紫杉醇脂质体的壳聚糖（核）/PCL-壳聚糖（壳）纳米纤维的性能进行了研究，以控制紫杉醇的释放和治疗乳腺癌。使用多分散指数、动态光散射、ζ电位、扫描电子显微镜、透射电子显微镜和傅里叶变换红外分析对合成配方进行了表征。载紫杉醇的纳米纤维在 30 天内实现了紫杉醇的持续释放。释放数据用 Korsmeyer-Peppas 药代动力学模型描述最佳。合成纳米纤维样品对 L929 正常细胞的细胞活力在 168 小时后高于 98%±1%。载脂质体的核壳纳米纤维对 MCF-7 乳腺癌细胞的最大细胞毒性为 85%±2.5%。体内结果表明，载脂质体的核壳纳米纤维可使肿瘤重量从 1.35±0.15g 减少至 0.65±0.05g，而纯核壳纳米纤维可使肿瘤重量从 1.35±0.15g 增加至 3.2±0.2g。载紫杉醇脂质体的核壳纳米纤维的三阶段药物释放行为和高体内肿瘤效率表明这些制剂将在未来用于癌症治疗。
