Institute of Biotechnology and Genetic Engineering, Bangabandhu Sheikh Mujibur Rahman Agricultural University, Gazipur, 1706, Bangladesh.
Department of Fisheries Management, Bangabandhu Sheikh Mujibur Rahman Agricultural University, Gazipur, 1706, Bangladesh.
Sci Rep. 2023 Jan 27;13(1):1551. doi: 10.1038/s41598-022-25968-8.
Enterococcus faecalis is associated with streptococcosis like infection in fish. A whole-genome sequence study was conducted to investigate the virulence factor and antibiotic-resistance genes in three fish pathogenic E. faecalis. Genomic DNA was extracted from three strains of E. faecalis isolated from streptococcosis infected Nile tilapia (strains BF1B1 and BFFF11) and Thai sarpunti (strain BFPS6). The whole genome sequences of these three strains were performed using a MiSeq sequencer (Illumina, Inc.). All three strains conserved 69 virulence factor such as genes associated with protection against oxidative stress, bacterial cell wall synthesis, gelatinase toxin, multiple biofilm-associated genes and capsule producing genes. Moreover, 39 antibiotic-resistance genes against sixteen major groups of antibiotics were identified in the genome sequences of all three strains. The most commonly used antibiotic Tetracycline resistance genes were found only in BFPS6 strain, whereas, Bacteriocin synthesis genes were identified in both BFFF11 and BFPS6 strain. Phylogenetic analysis revealed that strains BF1B1 and BFFF1 form a different cluster than BFPS6. This is one of the first whole-genome sequence study of fish pathogenic E. faecalis, unfold new information on the virulence factor and Antibiotic resistance genes linked to pathogenicity in fish.
粪肠球菌与鱼类的链球菌病样感染有关。本研究进行了全基因组序列分析，以研究三种鱼类致病性粪肠球菌中的毒力因子和抗生素耐药基因。从感染链球菌病的尼罗罗非鱼（BF1B1 和 BFFF11 株）和泰国萨普吞（BFPS6 株）中分离出的三株粪肠球菌提取基因组 DNA。使用 MiSeq 测序仪（Illumina，Inc.）对这三株的全基因组序列进行了测序。这三株均保守 69 种毒力因子，如与抗氧化应激、细菌细胞壁合成、明胶酶毒素、多种生物膜相关基因和荚膜产生基因相关的基因。此外，在这三株的基因组序列中还鉴定出了 39 种针对 16 大组抗生素的抗生素耐药基因。三种菌株都发现了最常用的抗生素四环素耐药基因，而细菌素合成基因则存在于 BFFF11 和 BFPS6 株中。系统发育分析表明，BF1B1 和 BFFF1 株与 BFPS6 株形成不同的聚类。这是鱼类致病性粪肠球菌全基因组序列研究之一，为鱼类致病性相关的毒力因子和抗生素耐药基因提供了新信息。
