通过 RHOH 表达和甲基化的综合分析预测肺腺癌的预后。
Prognostic prediction of lung adenocarcinoma by integrative analysis of RHOH expression and methylation.
机构信息
Phase I Clinical Trial Center, Fudan University Shanghai Cancer Center, Shanghai, China.
Department of Orthopaedic Oncology, Changzheng Hospital, Naval Military Medical University, Shanghai, China.
出版信息
Clin Respir J. 2023 Mar;17(3):148-156. doi: 10.1111/crj.13574. Epub 2023 Jan 29.
BACKGROUND AND OBJECTIVE
The development of epigenetics holds great promise for diagnosis and treatment of lung adenocarcinoma (LUAD). The purpose of this work was to analyze the correlation between Ras Homolog Gene Family Member H (RHOH) expression and methylation in patients with LUAD and its association with survival.
METHODS
Data related to gene expression, DNA methylation, and clinical features of LUAD from The Cancer Genome Atlas (TCGA) database were analyzed. A total of 50 patients were included in verification group. The methylation level of RHOH in verification group was detected by bisulfite amplicon sequencing.
RESULTS
The RHOH methylation level in TCGA cohort was significantly and negatively correlated with its expression level (Cor = -0.5, p = 2.687e-33). Patients with hypermethylation and low expression of RHOH had significantly worse prognosis than patients with hypomethylation and low expression of RHOH (TCGA: p = 0.004; validation cohort: p = 0.006, HR: 4.740, 95% CI: 1.567-14.340).
CONCLUSION
Our research revealed that RHOH may prove to be a new potential prognostic predictor for LUAD patients.
背景与目的
表观遗传学的发展为肺腺癌（LUAD）的诊断和治疗带来了巨大的希望。本研究旨在分析 LUAD 患者中 Ras Homolog Gene Family Member H（RHOH）表达与甲基化的相关性及其与生存的关系。
方法
分析来自癌症基因组图谱（TCGA）数据库的 LUAD 相关基因表达、DNA 甲基化和临床特征数据。验证组共纳入 50 例患者。通过亚硫酸氢盐扩增测序检测验证组中 RHOH 的甲基化水平。
结果
TCGA 队列中 RHOH 的甲基化水平与表达水平呈显著负相关（Cor = -0.5，p = 2.687e-33）。与 RHOH 低表达且低甲基化的患者相比，RHOH 高甲基化且低表达的患者预后明显更差（TCGA：p = 0.004；验证队列：p = 0.006，HR：4.740，95%CI：1.567-14.340）。
结论
我们的研究表明，RHOH 可能成为 LUAD 患者新的潜在预后预测指标。
Zotero 插件