腺嘌呤特异性DNA化学测序反应。

Iverson B L, Dervan P B

Division of Chemistry and Chemical Engineering, California Institute of Technology, Pasadena 91125.

Nucleic Acids Res. 1987 Oct 12;15(19):7823-30. doi: 10.1093/nar/15.19.7823.

Reaction of DNA with K2PdCl4 at pH 2.0 followed by a piperidine workup produces specific cleavage at adenine (A) residues. Product analysis revealed the K2PdCl4 reaction involves selective depurination at adenine, affording an excision reaction analogous to the other chemical DNA sequencing reactions. Adenine residues methylated at the exocyclic amine (N6) react with lower efficiency than unmethylated adenine in an identical sequence. This simple protocol specific for A may be a useful addition to current chemical sequencing reactions.

在pH 2.0条件下，DNA与K2PdCl4反应，随后进行哌啶后处理，可在腺嘌呤（A）残基处产生特异性切割。产物分析表明，K2PdCl4反应涉及腺嘌呤处的选择性脱嘌呤，产生一种类似于其他化学DNA测序反应的切除反应。在相同序列中，外环胺（N6）甲基化的腺嘌呤残基比未甲基化的腺嘌呤反应效率低。这种针对A的简单方案可能是对当前化学测序反应的有益补充。