Amesz Jorik H, Zhang Lu, Everts Bian R, De Groot Natasja M S, Taverne Yannick J H J
Translational Cardiothoracic Surgery Research Lab, Lowlands Institute for Bioelectric Medicine, Department of Cardiothoracic Surgery, Erasmus University Medical Center, Rotterdam, Netherlands.
Translational Electrophysiology, Lowlands Institute for Bioelectric Medicine, Department of Cardiology, Erasmus University Medical Center, Rotterdam, Netherlands.
Front Physiol. 2023 Jan 13;14:1076261. doi: 10.3389/fphys.2023.1076261. eCollection 2023.
Living myocardial slices (LMS) are ultrathin (150-400 µm) sections of intact myocardium that can be used as a comprehensive model for cardiac arrhythmia research. The recent introduction of biomimetic electromechanical cultivation chambers enables long-term cultivation and easy control of living myocardial slices culture conditions. The aim of this review is to present the potential of this biomimetic interface using living myocardial slices in electrophysiological studies outlining advantages, disadvantages and future perspectives of the model. Furthermore, different electrophysiological techniques and their application on living myocardial slices will be discussed. The developments of living myocardial slices in electrophysiology research will hopefully lead to future breakthroughs in the understanding of cardiac arrhythmia mechanisms and the development of novel therapeutic options.
活性心肌切片（LMS）是完整心肌的超薄（150 - 400微米）切片，可作为心律失常研究的综合模型。最近引入的仿生机电培养室能够对活性心肌切片进行长期培养，并易于控制其培养条件。本综述的目的是介绍这种使用活性心肌切片的仿生界面在电生理研究中的潜力，概述该模型的优缺点和未来前景。此外，还将讨论不同的电生理技术及其在活性心肌切片上的应用。活性心肌切片在电生理研究中的进展有望在未来对心律失常机制的理解以及新型治疗方案的开发方面带来突破。
