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用叠氮化钠处理种子以研究14个埃塞俄比亚芝麻(L.)基因型M2代的蒴果数量和质量性状。

Treatment of seeds with sodium azide for quantitative and qualitative capsule traits at M2 generation of Fourteen Ethiopian sesame ( L.) genotypes.

作者信息

Weldemichael Micheale Yifter, Baryatsion Yemane Tsehaye, Sbhatu Desta Berhe, Abraha Girmay Gebresamuel, Juhar Hagos Mohammedseid, Kassa Abraha Birhan, Sibhatu Fiseha Baraki, Gebremedhn Hailay Mehari, Mossa Mohammed Mebrahtu, Gebru Mullubrhan Mekonen, Meresa Birhanu Kahsay, Teklay Medhin, Berhe Birhanu Debesay, Gebru Haftay Abadi

机构信息

Department of Biotechnology, CDANR, Mekelle University, P.O. Box 231, Mekelle, Tigrai, Ethiopia.

Department of DCHS, CDANR, Mekelle University, P.O. Box 231, Mekelle, Tigrai, Ethiopia.

出版信息

Heliyon. 2023 Jan 10;9(1):e12912. doi: 10.1016/j.heliyon.2023.e12912. eCollection 2023 Jan.

Abstract

This study aimed at investigating the effects of sodium azide (NaN) on quantitative and qualitative capsule traits in M2 generation of 14 Ethiopian sesame genotypes collected from Humera Agricultural Research Center (HuARC), Tigrai. Both the treatment and control seeds were sown in well-prepared beds in greenhouse to develop M2 plants. Data on quantitative and qualitative traits were collected and analyzed using GenStat 16 software. Results showed significant differences among the M2 seeds treated with 0.75% NaN. The highest mean number of capsules per plant was recorded in ACC44 and Baha Necho genotypes, while the lowest was recorded in Gumero, Setit 2, Hirhir, ADI, Bounji and Aberghele. The highest mean number of seeds per capsule was recorded in Humera 1, Baha Necho, Zeri Tesfay, and Gondar 1 genotypes and the lowest was recorded in Setit 1, Setit 2 and ADI. The highest mean capsule length was observed in Zeri Tesfay while the lowest was recorded in Aberghele. The qualitative data reported that Hirhir, Setit 1 and Setit 2 were changed from completely shattering to partially shattering, Gumero and Bounji were changed from completely shattering to non-shattering, and Zeri Tesfay was changed from partially shattering to non-shattering. The 14 genotypes were clustered into four distinct groups including cluster I containing six genotypes, cluster II and III containing two genotypes each and cluster IV containing four genotypes. The mutants developed from Zeri Tesafy, ACC44 and Baha Necho genotypes are considered as potential candidate mutants for further utilization in sesame improvement.

摘要

本研究旨在调查叠氮化钠(NaN)对从提格雷州胡梅拉农业研究中心(HuARC)收集的14个埃塞俄比亚芝麻基因型M2代荚果数量和质量性状的影响。将处理过的种子和对照种子都播种在温室中精心准备的苗床里,以培育M2代植株。使用GenStat 16软件收集并分析了数量和质量性状的数据。结果显示,用0.75% NaN处理的M2代种子之间存在显著差异。ACC44和巴哈内乔基因型的单株荚果平均数最高,而古梅罗、塞蒂特2、希尔希尔、阿迪、邦吉和阿贝盖勒的单株荚果平均数最低。胡梅拉1、巴哈内乔、泽里·特斯法伊和贡德尔1基因型的每荚种子平均数最高,而塞蒂特1、塞蒂特2和阿迪的每荚种子平均数最低。泽里·特斯法伊的平均荚果长度最长,而阿贝盖勒的平均荚果长度最短。定性数据表明,希尔希尔、塞蒂特1和塞蒂特2从完全裂蒴变为部分裂蒴,古梅罗和邦吉从完全裂蒴变为不裂蒴,泽里·特斯法伊从部分裂蒴变为不裂蒴。这14个基因型被聚类为四个不同的组,包括包含六个基因型的第一组、每组包含两个基因型的第二组和第三组以及包含四个基因型的第四组。从泽里·特萨菲、ACC44和巴哈内乔基因型培育出的突变体被认为是芝麻改良中进一步利用的潜在候选突变体。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/03a9/9876834/2f2d46270688/gr1.jpg

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