Shlosberg Yaniv, Limwongyut Jakkarin, Moreland Alex S, Bazan Guillermo C
Department of Chemistry and Biochemistry, University of California at Santa Barbara, Santa Barbara, CA, 93106, United States.
bioRxiv. 2023 Jan 19:2023.01.16.524302. doi: 10.1101/2023.01.16.524302.
In recent years, the concern from the global climate change has driven an urgent need to develop clean energy technologies that do not involve combustion process that emit carbon into the atmosphere. A promising concept is microbial fuel cells that utilize bacteria as electron donors in a bio-electrochemical cell performing a direct electron transfer via conductive protein complexes or by secretion of redox active metabolites such as quinone or phenazine derivatives. In the case of photosynthetic bacteria (cyanobacteria) electrons can also be extracted from the photosynthetic pathway mediated mostly by NADH and NADPH. In this work, we show for the first time that the intact non-photosynthetic bacteria can produce photocurrent that is enhanced upon addition of an exogenous electron mediator. Furthermore, we apply 2D-fluorescence measurement to show that NADH is released from the bacterial cells, which may apply as a native electron mediator in microbial fuel cells.
近年来，全球气候变化引发的担忧促使人们迫切需要开发不涉及向大气排放碳的燃烧过程的清洁能源技术。一个有前景的概念是微生物燃料电池，它在生物电化学电池中利用细菌作为电子供体，通过导电蛋白复合物或分泌醌或吩嗪衍生物等氧化还原活性代谢物进行直接电子转移。对于光合细菌（蓝细菌），电子也可以从主要由NADH和NADPH介导的光合途径中提取。在这项工作中，我们首次表明完整的非光合细菌可以产生光电流，并且在添加外源电子介质后光电流会增强。此外，我们应用二维荧光测量来表明NADH从细菌细胞中释放出来，这可能作为微生物燃料电池中的天然电子介质。
