Li Yao, Gao Xinchang, Pan Daodong, Liu Zhu, Xiao Chaogeng, Xiong Yongzhao, Du Lihui, Cai Zhendong, Lu Wenjing, Dang Yali, Zhu Xiuzhi
State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of AgroProducts, College of Food and Pharmaceutical Sciences, Ningbo University, Ningbo, Zhejiang, China.
Department of Chemistry, Tsinghua University, Beijing, China.
Front Nutr. 2023 Jan 11;9:1118900. doi: 10.3389/fnut.2022.1118900. eCollection 2022.
fermentation of broccoli as a good source of bioactive peptides has not been fully elucidated. In this work, the peptide composition of broccoli fermented by A3 and ATCC7469 was analyzed by peptidomics to study the protein digestion patterns after fermentation by different strains. Results showed that water-soluble proteins such as rubisco were abundant sources of peptides, which triggered the sustained release of peptides as the main target of hydrolysis. In addition, 17 novel anti-inflammatory peptides were identified by virtual screening. Among them, SIWYGPDRP had the strongest ability to inhibit the release of NO from inflammatory cells at a concentration of 25 μM with an inhibition rate of 52.32 ± 1.48%. RFR and KASFAFAGL had the strongest inhibitory effects on the secretion of TNF-α and IL-6, respectively. At a concentration of 25 μM, the corresponding inhibition rates were 74.61 ± 1.68% and 29.84 ± 0.63%, respectively. Molecular docking results showed that 17 peptides formed hydrogen bonds and hydrophobic interactions with inducible nitric oxide synthase (iNOS). This study is conducive to the high-value utilization of broccoli and reduction of the antibiotic use.
西兰花作为生物活性肽的优质来源，其发酵过程尚未得到充分阐释。在本研究中，采用肽组学方法分析了由A3和ATCC7469发酵的西兰花的肽组成，以研究不同菌株发酵后蛋白质的消化模式。结果表明，诸如核酮糖-1,5-二磷酸羧化酶等水溶性蛋白质是肽的丰富来源，这些蛋白质引发了肽的持续释放，作为水解的主要靶点。此外，通过虚拟筛选鉴定出17种新型抗炎肽。其中，SIWYGPDRP在浓度为25 μM时具有最强的抑制炎症细胞释放NO的能力，抑制率为52.32±1.48%。RFR和KASFAFAGL分别对TNF-α和IL-6的分泌具有最强的抑制作用。在浓度为25 μM时，相应的抑制率分别为74.61±1.68%和29.84±0.63%。分子对接结果表明，17种肽与诱导型一氧化氮合酶（iNOS）形成了氢键和疏水相互作用。本研究有助于西兰花的高值化利用并减少抗生素的使用。
