Hunan Provincial Key Laboratory of Cytochemistry, School of Food Science and Bioengineering, Changsha University of Science & Technology, Changsha, 410114, China.
Plant Foods Hum Nutr. 2022 Jun;77(2):172-180. doi: 10.1007/s11130-022-00963-6. Epub 2022 Apr 21.
Inflammation is a contributing factor to the initiation and progression of many diseases, and some food-derived biofunctional peptides show high anti-inflammatory activity. In our previous study, we demonstrated that peptides derived from trypsin hydrolysis of rice protein show good immunological activity. In the present study, proteins of broken rice were extracted and identified by macroporous resin fractionation and liquid chromatography/tandem mass spectrometry (LC-MS/MS). Subsequently, a bioinformatics prediction and in silico simulation approach was used to screen for peptides showing anti-inflammatory activity, including inhibition of the production of nitric oxide and proinflammatory cytokines (interleukin-1β, interleukin-6, and tumor necrosis factor-α) by lipopolysaccharide-stimulated RAW264.7 mice macrophages. Three peptides (DNIQGITKPAIR, IAFKTNPNSMVSHIAGK, and IGVAMDYSASSKR) that demonstrated the highest binding affinity were synthesized, and their in vitro anti-inflammatory activity was investigated. This is the first study that integrates LC-MS/MS identification and bioinformatics prediction for reporting the anti-inflammatory activity of anti-inflammatory peptides derived from broken rice protein. The study findings revealed that the peptides derived from the byproduct of rice milling could be potentially used as natural anti-inflammatory alternativities.
炎症是许多疾病发生和发展的一个因素，一些来源于食物的生物活性肽显示出很高的抗炎活性。在我们之前的研究中，我们证明了来源于米蛋白的胰蛋白酶水解肽具有很好的免疫活性。在本研究中，通过大孔树脂分级和液相色谱/串联质谱（LC-MS/MS）对碎米蛋白进行了提取和鉴定。随后，采用生物信息学预测和计算机模拟方法筛选出具有抗炎活性的肽，包括抑制脂多糖刺激的 RAW264.7 小鼠巨噬细胞产生一氧化氮和促炎细胞因子（白细胞介素-1β、白细胞介素-6 和肿瘤坏死因子-α）。合成了具有最高结合亲和力的三个肽（DNIQGITKPAIR、IAFKTNPNSMVSHIAGK 和 IGVAMDYSASSKR），并研究了它们的体外抗炎活性。这是首次将 LC-MS/MS 鉴定和生物信息学预测相结合，报道来源于碎米蛋白的抗炎肽的抗炎活性的研究。研究结果表明，来源于稻米加工副产物的肽可能可作为天然抗炎替代品。
