Panase Arporn, Thirabunyanon Mongkol, Promya Jongkon, Chitmanat Chanagun
Program in Biotechnology, Faculty of Science, Maejo University, Chiang Mai, Thailand.
Faculty of Fisheries Technology and Aquatic Resources, Maejo University, Chiang Mai, Thailand.
Front Vet Sci. 2023 Jan 12;9:1094681. doi: 10.3389/fvets.2022.1094681. eCollection 2022.
The present study investigated the effects of and fructooligosaccharide (FOS) on growth performances, immunity improvement, and disease resistance of Nile tilapia (). The fish (24.5 ± 1.6 g) were fed a basal diet (G1), diets supplemented with 1 g/kg (G2), 3 g/kg (G3) and 5 g/kg (G4) of FOS as well as diets supplemented with 1 × 10 CFU/g (G5), 3 × 10 CFU/g (G6) and 5 × 10 CFU/g (G7) of for 56 days. After the feeding trial, the complement C3, β, α, IFN-γ, gene expression in the liver was then analyzed by a quantitative Real-time PCR. Then, fish were infected with , and the survival rate was recorded. The results showed that FOS and had no significant effect ( > 0.05) on growth performances and survival rate. Lysozyme activity was significantly greater in the G4, G5, G6, and G7 groups. Also, all fish fed FOS and showed significantly ( < 0.05) higher respiratory burst activity than other groups. The expressions of complement β, α, γ, and in the liver were significantly higher for fish fed 5 g/kg of FOS as well as for fish that received any concentration level of ( < 0.05) used in the study. After the challenge test, the survival rate of fish-fed diets supplemented with FOS and was slightly higher than for the control group. The results indicated that FOS an could stimulate immune responses and immune-related genes in tilapia. However, further investigation of other prebiotics or herbs in combination with is encouraged at molecular levels and screening for beneficial metabolites that may increasingly improve digestive enzymes, growth performances, and health benefits in tilapia. In addition, on-farm experiments are needed.
本研究调查了低聚果糖（FOS）和[具体物质未给出英文名称]对尼罗罗非鱼生长性能、免疫改善及抗病能力的影响。将体重为（24.5±1.6克）的鱼分为以下几组投喂：基础饲料组（G1）、添加1克/千克FOS的饲料组（G2）、添加3克/千克FOS的饲料组（G3）、添加5克/千克FOS的饲料组（G4）、添加1×10[具体CFU/g数值未给出]CFU/克[具体物质未给出英文名称]的饲料组（G5）、添加3×10[具体CFU/g数值未给出]CFU/克[具体物质未给出英文名称]的饲料组（G6）以及添加5×10[具体CFU/g数值未给出]CFU/克[具体物质未给出英文名称]的饲料组（G7），投喂56天。饲养试验结束后，通过定量实时PCR分析肝脏中补体C3、β、α、干扰素-γ、[具体基因未给出英文名称]基因的表达。然后，用[具体病原体未给出英文名称]感染鱼，并记录存活率。结果表明，FOS和[具体物质未给出英文名称]对生长性能和存活率无显著影响（P>0.05）。G4、G5、G6和G7组的溶菌酶活性显著更高。此外，所有投喂FOS和[具体物质未给出英文名称]的鱼的呼吸爆发活性均显著高于其他组（P<0.05）。对于投喂5克/千克FOS的鱼以及接受本研究中任何浓度水平[具体物质未给出英文名称]的鱼，肝脏中补体β、α、γ和[具体基因未给出英文名称]的表达均显著更高（P<0.05）。在[具体病原体未给出英文名称]攻毒试验后，投喂添加FOS和[具体物质未给出英文名称]饲料的鱼的存活率略高于对照组。结果表明，FOS和[具体物质未给出英文名称]可刺激罗非鱼的免疫反应和免疫相关基因。然而，鼓励在分子水平上进一步研究其他益生元或草药与[具体物质未给出英文名称]的组合，并筛选可能更多地改善罗非鱼消化酶、生长性能和健康效益的有益代谢产物。此外，还需要进行农场试验。
