Zhang Qin, Yu Hairui, Tong Tong, Tong Wanping, Dong Lanfang, Xu Mingzhu, Wang Zhicheng
Key Laboratory of Marine Biotechnology of Guangxi, Guangxi Institute of Oceanology, 92 Changqing Road East, Beihai, Guangxi 536000, PR China.
Key Laboratory of Biochemistry and Molecular Biology in Universities of Shandong (Weifang University), College of Biological and Agricultural Engineering, Weifang University, Tianrun Bioengineering R & D Center of Weifang New and High-tech Zone, Weifang 261061, PR China.
Fish Shellfish Immunol. 2014 May;38(1):7-14. doi: 10.1016/j.fsi.2014.02.008. Epub 2014 Mar 12.
A feeding trial was conducted to investigate the effects of dietary administration of probiotic Bacillus subtilis and prebiotic fructooligosaccharide (FOS) on growth performance, immune responses and disease resistance of juvenile ovate pompano, Trachinotus ovatus. One thousand six hundred and twenty individuals (initial body weight: 10.32 ± 0.46 g, mean ± S.E) were fed nine practical diets according to a 3 × 3 factorial design: the basal diet as the control diet supplemented with three levels of B. subtilis (0, 1.05 × 10(7) or 5.62 × 10(7) CFU g(-1) diet), crossed with 0, 0.2% or 0.4% FOS. After an 8-week feeding experimental period, six fish per cage were sampled for immunity determination. Then 18 fish of each cage left were challenged by Vibrio vulnificus. The results showed that fish fed with 5.62 × 10(7) CFU B. subtilis g(-1) in combination with 0.2% FOS produced the highest specific growth rate, and were significantly higher than the groups fed with 0 and 0.2% FOS without B. subtilis supplementation (P < 0.05). Feed efficiency ratio significantly increased with the increasing doses of dietary FOS without B. subtilis added (P < 0.05). The immune assay showed that fish fed with the control diet produced the lowest respiratory burst activity and was significantly different from the groups fed the diets containing 0.2% FOS at each B. subtilis level and containing 0.4% FOS single (P < 0.05). Phagocytic activity was significantly decreased with the increasing doses of dietary B. subtilis at 0.4% FOS level (P < 0.05). Alternative complement pathway activity of the fish fed with 0.2% FOS single was significantly lower than those fed with 5.62 × 10(7) CFU B. subtilis g(-1) diet supplemented at each FOS level (P < 0.05). Fish fed with the control diet had the lowest lysozyme activity, and were significantly different from those fed with 0.2 or 0.4% FOS at 1.05 and 5.62 × 10(7) CFU B. subtilis g(-1) diet level. Moreover, fish fed with diets supplemented with 0.2% and 0.4% FOS at each B. subtilis level had notably lower cumulative mortality after 10 days following V. vulnificus infection (P < 0.05). Under the experimental conditions, dietary B. subtilis and FOS had a significant interaction on enhancing the immune responses and disease resistance of juvenile ovate pompano (P < 0.05).
进行了一项饲养试验，以研究日粮中添加益生菌枯草芽孢杆菌和益生元低聚果糖（FOS）对卵形鲳鲹幼鱼生长性能、免疫反应和抗病能力的影响。1620尾个体（初始体重：10.32±0.46克，平均值±标准误）按照3×3析因设计投喂九种实用日粮：基础日粮作为对照日粮，添加三个水平的枯草芽孢杆菌（0、1.05×10⁷或5.62×10⁷CFU g⁻¹日粮），与0、0.2%或0.4%的FOS交叉组合。经过8周的饲养试验期后，每个网箱取6尾鱼进行免疫测定。然后对每个网箱剩下的18尾鱼用创伤弧菌进行攻毒。结果表明，日粮中添加5.62×10⁷CFU枯草芽孢杆菌g⁻¹并搭配0.2%FOS的鱼体特定生长率最高，显著高于未添加枯草芽孢杆菌的0和0.2%FOS组（P<0.05）。在不添加枯草芽孢杆菌的情况下，饲料效率随着日粮中FOS剂量的增加而显著提高（P<0.05）。免疫分析表明，投喂对照日粮的鱼呼吸爆发活性最低，与在每个枯草芽孢杆菌水平下投喂含0.2%FOS日粮的组以及单独投喂0.4%FOS日粮的组有显著差异（P<0.05）。在FOS水平为0.4%时，吞噬活性随着日粮中枯草芽孢杆菌剂量的增加而显著降低（P<0.05）。单独投喂0.2%FOS的鱼的替代补体途径活性显著低于在每个FOS水平下添加5.62×10⁷CFU枯草芽孢杆菌g⁻¹日粮的组（P<0.05）。投喂对照日粮的鱼溶菌酶活性最低，与在1.05和5.62×10⁷CFU枯草芽孢杆菌g⁻¹日粮水平下投喂0.2%或0.4%FOS的组有显著差异。此外，在每个枯草芽孢杆菌水平下，投喂添加0.2%和0.4%FOS日粮的鱼在创伤弧菌感染10天后的累积死亡率显著较低（P<0.05）。在试验条件下，日粮中的枯草芽孢杆菌和FOS在增强卵形鲳鲹幼鱼免疫反应和抗病能力方面存在显著交互作用（P<0.05）。
