Wei Yu-Rong, Ma Wen-Ge, Wang Ping, Wang Wen, Su Xiao-Hui, Yang Xue-Yun, Mi Xiao-Yun, Wu Jian-Yong, Huang Jiong
Xinjiang Key Laboratory of Animal Infectious Diseases, Institute of Veterinary Medicine, Xinjiang Academy of Animal Science, Urumqi, China.
Center for Animal Disease Prevention and Control of Xinjiang, Urumqi, China.
Front Vet Sci. 2023 Jan 12;9:1073648. doi: 10.3389/fvets.2022.1073648. eCollection 2022.
Lumpy skin disease caused by Lumpy skin disease virus (LSDV) is a severe systemic disease affecting cattle and other ruminants. Lumpy skin disease was first reported in northwest China in August 2019 and has severely threatened the cattle breeding industry in China. However, there have been limited genomic studies of LSDV from the first outbreak and its subsequent epidemics. This study aims to characterize the comparative genomic evolution of the LSDV strain from the first outbreak in China. The etiological agent was isolated in a Madin-Darby bovine kidney cell culture and subsequently identified by PCR and Sanger sequencing of six selected genes. The genome sequence was determined using Illumina sequencing and analyzed through genome alignment and phylogenetic tree. The results showed that all six genes were successfully amplified and genetically clustered into LSDV. The virus presented the highest homology to strain China/GD01/2020, which shared 100% identities among 150 open reading frames (ORFs), and 97.1-99.7% identities among additional 6 ORFs. Bayesian inference tree analysis revealed that the virus shared a common ancestor with LSDV strains from China and Vietnam. The study provides an additional genomic data for LSDV tracking and control in China and neighboring countries.
由结节性皮肤病病毒（LSDV）引起的结节性皮肤病是一种影响牛和其他反刍动物的严重全身性疾病。结节性皮肤病于2019年8月首次在中国西北部被报道，严重威胁了中国的养牛业。然而，对于首次疫情及其后续流行中的LSDV的基因组研究有限。本研究旨在表征来自中国首次疫情的LSDV毒株的比较基因组进化。病原体在Madin-Darby牛肾细胞培养物中分离，随后通过对六个选定基因进行PCR和桑格测序进行鉴定。使用Illumina测序确定基因组序列，并通过基因组比对和系统发育树进行分析。结果表明，所有六个基因均成功扩增并在基因上聚类为LSDV。该病毒与毒株China/GD01/2020具有最高的同源性，在150个开放阅读框（ORF）中共享100%的同一性，在另外6个ORF中共享97.1-99.7%的同一性。贝叶斯推断树分析表明，该病毒与来自中国和越南的LSDV毒株有共同的祖先。该研究为中国及周边国家的LSDV追踪和控制提供了额外的基因组数据。
