Alkayali Mohamad Fathi Mohamad Taher, Badria Farid A, ElBaiomy Azza Abdel Baky, Youssef Jilan Mohamed
Department of Oral Medicine, Periodontology, Diagnosis and Oral Radiology, Faculty of Dentistry, Mansoura University, Mansoura, Egypt.
Department of Pharmacognosy, Faculty of Pharmacy, Mansoura University, Mansoura, Egypt.
J Adv Periodontol Implant Dent. 2022 Nov 20;14(2):76-83. doi: 10.34172/japid.2022.024. eCollection 2022.
The aim of this study was the clinical and laboratory evaluation of using polycaprolactone (PCL) nanofibers loaded with oxytetracycline hydrochloride (OTC) and zinc oxide (ZnO) as an adjunct to mechanical therapy in managing stage II grade A periodontitis patients concerning GCF lipocalin2- levels. Fifty stage II grade A periodontitis patients (27 women and 23 men, with an age range of 30‒60) were enrolled in the study. The patients were randomly assigned to five equal groups and treated with scaling and root planing (SRP), followed by local application of PCL nanofibers: group I: SRP + PCL loaded with OTC and ZnO, group II: SRP + PCL loaded with OTC, group III: SRP + PCL loaded with ZnO, group IV: SRP + unloaded PCL, and group V: SRP alone. Additionally, 10 healthy subjects with healthy periodontium (group VI) (age- and gender-matched) served as the negative control. Nanofibers were applied in the selected pockets of periodontitis patients in groups I to IV once weekly for two months. All the participants were examined clinically by assessing periodontal indices (gingival index, plaque index, pocket depth, and clinical attachment level), and biochemically by assessing GCF lipocalin-2 levels. Compared to controls, periodontitis groups (I, II, III, IV, and V) showed significant elevation of both clinical parameters and GCF lipocalin2- levels at baseline. In addition, these parameters improved significantly after treatment, which was more pronounced in groups I, II-, and III) compared to groups IV and V. However, it did not reach normal values. In association with SRP, PCL nanofibers loaded with OTC and ZnO had beneficial therapeutic effects at both clinical and laboratory levels.
本研究的目的是对使用负载盐酸土霉素（OTC）和氧化锌（ZnO）的聚己内酯（PCL）纳米纤维作为机械治疗的辅助手段来治疗II期A级牙周炎患者时，龈沟液中lipocalin2水平进行临床和实验室评估。50例II期A级牙周炎患者（27名女性和23名男性，年龄范围为30 - 60岁）被纳入研究。患者被随机分为五组，接受龈上洁治和根面平整（SRP），随后局部应用PCL纳米纤维：第一组：SRP + 负载OTC和ZnO的PCL，第二组：SRP + 负载OTC的PCL，第三组：SRP + 负载ZnO的PCL，第四组：SRP + 未负载的PCL，第五组：仅SRP。此外，10名牙周健康的受试者（第六组）（年龄和性别匹配）作为阴性对照。纳米纤维每周一次应用于I至IV组牙周炎患者的选定牙周袋，持续两个月。所有参与者均通过评估牙周指数（牙龈指数、菌斑指数、牙周袋深度和临床附着水平）进行临床检查，并通过评估龈沟液中lipocalin - 2水平进行生化检查。与对照组相比，牙周炎组（I、II、III、IV和V）在基线时临床参数和龈沟液中lipocalin2水平均显著升高。此外，治疗后这些参数有显著改善，与IV组和V组相比，I、II和III组更为明显。然而，这些参数未达到正常值。与SRP联合使用时，负载OTC和ZnO的PCL纳米纤维在临床和实验室水平均具有有益的治疗效果。
