Restorative Dentistry Department, Faculty of Dentistry, Universidade Federal de Minas Gerais, Av. Presidente Antônio Carlos 6627, Belo Horizonte, MG CEP 31270901, Brazil.
Chemistry Department, Instituto de Ciências Exatas, Universidade Federal de Minas Gerais, Av. Presidente Antônio Carlos 6627, Belo Horizonte, MG CEP 31270901, Brazil.
Mater Sci Eng C Mater Biol Appl. 2019 Oct;103:109798. doi: 10.1016/j.msec.2019.109798. Epub 2019 May 27.
Periodontal diseases (PD) are mixed bacterial infections caused by microorganisms that colonize the tooth surface, leading to destructions at tooth-supporting tissues. Several local delivery systems, as nanofibers, have been developed for the treatment of PD. The purpose of the present study was developing polycaprolactone (PCL) nanofibers incorporating two antibacterial agents, OTC and ZnO, for use in the treatment of PD. Nanofibers were produced by electrospinning method: PCL loaded with ZnO (PCL-Z), PCL loaded with OTC (PCL-OTC), PCL loaded with OTC and ZnO (PCL-OTCz) and pristine PCL (PCL-P). The nanofibers were characterized physicochemically using different techniques. In addition, in vitro study of the OTC release from the nanofibers was performed. The PCL-OCT showed sustained release of the drug up to 10 h, releasing 100% of OTC. However, the PCL-OTCz nanofiber showed a slow release of OTC up to 120 h (5th day) with 54% of drug retention. The cytotoxicity assay showed that PCL-OTC nanofiber was slightly cytotoxic after 48 h and the other nanofibers were non-cytotoxic. The antibacterial activity of the nanofibers was evaluated by qualitative and quantitative analysis and against mixed bacterial culture, composed of four Gram-negative anaerobic bacteria involved in periodontal diseases. The disk diffusion method showed that the PCL-OTC displayed higher inhibition zone than PCL-OTCz (p < 0.001). The quantitative analysis, evaluated by broth culture, showed that the PCL-OTC and PCL-OTCz exhibited excellent activity against a mixed bacterial culture with growth inhibition of 98.0% and 97.5%, respectively. Based on these results, the PCL-OTCz nanofibers developed have great potential as a drug delivery system for the PD treatment.
牙周病（PD）是由定植在牙面的微生物引起的混合细菌感染，导致牙齿支持组织的破坏。已经开发了几种局部递送系统，如纳米纤维，用于治疗 PD。本研究的目的是开发包含两种抗菌剂 OTC 和 ZnO 的聚己内酯（PCL）纳米纤维，用于治疗 PD。纳米纤维通过静电纺丝法制备：负载 ZnO 的 PCL（PCL-Z）、负载 OTC 的 PCL（PCL-OTC）、负载 OTC 和 ZnO 的 PCL（PCL-OTCz）和纯 PCL（PCL-P）。使用不同的技术对纳米纤维进行了物理化学表征。此外，还进行了纳米纤维中 OTC 释放的体外研究。PCL-OCT 显示药物持续释放长达 10 小时，释放 100%的 OTC。然而，PCL-OTCz 纳米纤维显示 OTC 缓慢释放，直到 120 小时（第 5 天），保留 54%的药物。细胞毒性试验表明，PCL-OTC 纳米纤维在 48 小时后略有细胞毒性，而其他纳米纤维无细胞毒性。通过定性和定量分析以及对混合细菌培养物（由四种参与牙周病的革兰氏阴性厌氧菌组成）评估纳米纤维的抗菌活性。圆盘扩散法显示 PCL-OTC 显示出比 PCL-OTCz 更高的抑制带（p<0.001）。通过肉汤培养评估的定量分析表明，PCL-OTC 和 PCL-OTCz 对混合细菌培养物表现出优异的活性，生长抑制率分别为 98.0%和 97.5%。基于这些结果，开发的 PCL-OTCz 纳米纤维具有作为 PD 治疗的药物递送系统的巨大潜力。
