Seo Nuri, Park Chan, Stahl Alexander M, Cho Hoonsung, Park Sang-Won, Yim Soon-Ho, Yun Kwi-Dug, Ji Min-Kyung, Kim Heesun, Yang Yunzhi Peter, Lim Hyun-Pil
Department of Prosthodontics, School of Dentistry, Chonnam National University, Gwangju 61186, Korea.
Department of Chemistry, Stanford University, Stanford, California 94305, United States.
J Nanosci Nanotechnol. 2021 Jul 1;21(7):3683-3688. doi: 10.1166/jnn.2021.19156.
During the design of membranes for guided tissue regeneration (GTR) to treat periodontal diseases, infection of the exposed membranes and postoperative complications can be prevented by increasing bacterial resistance. This study evaluated the antibacterial activity of PCL/ZnO membranes and their effect on cell viability via addition of antibacterial zinc oxide (ZnO) nanoparticles to a biocompatible and biodegradable material such as polycaprolactone (PCL). Neat PCL membranes and PCL/ZnO membranes containing 0.5 wt.% and 5 wt.% ZnO were produced, and divided into PCL (0% ZnO), LZ (0.5 wt.% ZnO), and HZ (5 wt.% ZnO) groups, respectively. The surface characteristics of the membranes including morphological features and changes in composition were analyzed. Adhesion of bacteria, including and was analyzed using a crystal violet assay. The proliferation of MC3T3-E1 osteoblasts was evaluated using a WST-8 assay. Significant differences were analyzed using the Kruskal-Wallis test (P < 0.05). The results of groups were compared using the Mann-Whitney test ( < 0.017). ZnO nanoparticles were dispersed in the PCL matrix of PCL/ZnO membranes. Compared with neat PCL membranes, their ability to form crystals decreased and their amorphous structure increased. The adhesion of S. and in the LZ and HZ groups containing ZnO was significantly decreased compared with that of the neat PCL membranes (P < 0.05). No significant differences were observed in the proliferation of MC3T3-E1 cells between the PCL/ZnO membranes and the neat PCL membranes both on days 2 and 5 of culture (P > 0.05). This study has demonstrated that the PCL membranes carrying the ZnO nanoparticles inhibited bacterial adhesion without affecting the viability of osteoblasts, suggesting the potential application of ZnO in GTR to increase antibacterial activity of membranes.
在设计用于治疗牙周疾病的引导组织再生（GTR）膜时，可通过增强细菌抗性来预防暴露膜的感染和术后并发症。本研究通过向聚己内酯（PCL）这种生物相容性和可生物降解材料中添加抗菌氧化锌（ZnO）纳米颗粒，评估了PCL/ZnO膜的抗菌活性及其对细胞活力的影响。制备了纯PCL膜以及含有0.5 wt.%和5 wt.% ZnO的PCL/ZnO膜，并分别分为PCL（0% ZnO）、LZ（0.5 wt.% ZnO）和HZ（5 wt.% ZnO）组。分析了膜的表面特性（包括形态特征和成分变化）。使用结晶紫测定法分析了包括[具体细菌名称1]和[具体细菌名称2]在内的细菌的黏附情况。使用WST - 8测定法评估了MC3T3 - E1成骨细胞的增殖情况。使用Kruskal - Wallis检验分析显著差异（P < 0.05）。使用Mann - Whitney检验比较组间结果（P < 0.017）。ZnO纳米颗粒分散在PCL/ZnO膜的PCL基质中。与纯PCL膜相比，它们形成晶体的能力降低，非晶结构增加。与纯PCL膜相比，含ZnO的LZ组和HZ组中[具体细菌名称1]和[具体细菌名称2]的黏附显著降低（P < 0.05）。在培养的第2天和第5天，PCL/ZnO膜与纯PCL膜之间的MC3T3 - E1细胞增殖未观察到显著差异（P > 0.05）。本研究表明，携带ZnO纳米颗粒的PCL膜可抑制细菌黏附，而不影响成骨细胞的活力，这表明ZnO在GTR中增加膜的抗菌活性方面具有潜在应用价值。
