Lin Wenjia, Liu Shiying, Huang Zhuojian, Li Haiwen, Lu Tianyu, Luo Yongxin, Zhong Jiamin, Xu Zewen, Liu Yu, Li Yanwu, Li Peiwu, Xu Qian, Cai Jiazhong, Li Huibiao, Chen Xin-Lin
School of Basic Medical Sciences, Guangzhou University of Chinese Medicine, Guangzhou, China.
Shenzhen Traditional Chinese Medicine Hospital, Shenzhen, China.
Front Med (Lausanne). 2023 Jan 12;9:1064106. doi: 10.3389/fmed.2022.1064106. eCollection 2022.
For Crohn's disease (CD), the alternation of the active phase and inactive phase may be related to humoral immunity and cellular immunity. This study aims to understand the characteristics of immune cells in patients with active CD (CDa) and inactive CD (CDin).
Mass cytometry (CyTOF) and single-cell RNA sequencing (scRNA-seq) data about CDa, CDin, and healthy control (HC) were included. CyTOF analysis was performed to capture gated subsets, including T cells, T regulatory (Treg) cells, B cells, innate immune cells, and natural killer (NK) cells. Differential analysis was used to identify different immune cell subsets among CDa, CDin, and HC. ScRNA-seq analysis was used to verify the results of CyTOF. CD-related signaling pathways were obtained using KEGG pathway enrichment analysis. CellChat analysis was used to infer the cell communication network among immune cell subsets.
Compared to patients with CDin, patients with CDa had higher abundances of CD16CD38CD4CXCR3CCR6 naive T cells, HLA-DRCD38IFNγTNF effector memory (EM) T cells, HLA-DRIFNγ naive B cells, and CD14CD11CIFNγIL1B monocytes. KEGG analysis showed the similarity of pathway enrichment for the earlier four subsets, such as thermogenesis, oxidative phosphorylation, and metabolic pathways. The patients with CDin were characterized by an increased number of CD16CD56CD44HLA-DRIL22 NK cells. Compared to HC, patients with CDa demonstrated a low abundance of HLA-DRCCR6 NK cells and a high abundance of FOXP3CD44 EM Tregs. CellChat analysis revealed the interaction network of cell subsets amplifying in CDa compared with CDin.
Some immune subsets cells were identified for CDa and CDin. These cells may be related to the occurrence and development of CD and may provide assistance in disease diagnosis and treatment.
对于克罗恩病（CD），其活动期与非活动期的交替可能与体液免疫和细胞免疫有关。本研究旨在了解活动期CD（CDa）和非活动期CD（CDin）患者免疫细胞的特征。
纳入有关CDa、CDin和健康对照（HC）的质谱流式细胞术（CyTOF）和单细胞RNA测序（scRNA-seq）数据。进行CyTOF分析以捕获门控亚群，包括T细胞、调节性T（Treg）细胞、B细胞、固有免疫细胞和自然杀伤（NK）细胞。采用差异分析来鉴定CDa、CDin和HC之间不同的免疫细胞亚群。使用scRNA-seq分析来验证CyTOF的结果。通过KEGG通路富集分析获得与CD相关的信号通路。使用CellChat分析来推断免疫细胞亚群之间的细胞通讯网络。
与CDin患者相比，CDa患者的CD16CD38CD4CXCR3CCR6初始T细胞、HLA-DRCD38IFNγTNF效应记忆（EM）T细胞、HLA-DRIFNγ初始B细胞和CD14CD11CIFNγIL1B单核细胞丰度更高。KEGG分析显示前四个亚群的通路富集相似，如产热、氧化磷酸化和代谢途径。CDin患者的特征是CD16CD56CD44HLA-DRIL22 NK细胞数量增加。与HC相比，CDa患者的HLA-DRCCR6 NK细胞丰度较低，而FOXP3CD44 EM Tregs丰度较高。CellChat分析揭示了与CDin相比在CDa中扩增的细胞亚群的相互作用网络。
鉴定出了一些与CDa和CDin相关的免疫亚群细胞。这些细胞可能与CD的发生和发展有关，并可能为疾病的诊断和治疗提供帮助。
