Liang Jin, Hu Yuanfang, Fu Mingxia, Li Na, Wang Fengxia, Yu Xiaojun, Ji Bing
Department of Laboratory Medicine, Binzhou Medical University Hospital, Binzhou, People's Republic of China.
Department of Laboratory Medicine, Binzhou People's Hospital, Binzhou, People's Republic of China.
Infect Drug Resist. 2023 Jan 23;16:379-388. doi: 10.2147/IDR.S392908. eCollection 2023.
To investigate the molecular epidemiology of () isolated from patients in the Yellow River Delta region and to construct antibiotic resistance profiles in different genetic backgrounds.
Antibiotics susceptibility testing, staphylococcal protein A () typing and accessory gene regulator () typing were performed for all the 204 strains. Isolates with the positive gene and heterogeneous vancomycin-intermediate (hVISA) were subjected to multilocus sequence typing (MLST) and Staphylococcal chromosomal cassettes (SCC) typing.
Thirty-nine MRSA strains were identified by gene. Twenty-two hVISA isolates including 9 MRSA and 13 methicillin-sensitive (MSSA) strains were confirmed, the rest isolates (n = 182) were vancomycin-susceptible (VSSA). 94.9% of MRSA and 63.6% of hVISA belonged to I. Fifty-seven distinct types including 5 novel types were mainly t309 (30.9%), t078 (11.8%) and t437 (11.8%). Fourteen sequence types (STs) containing 3 new STs were classified into 3 clone complexes (CCs) and 7 singletons among MRSA and hVISA isolates. Most MRSA isolates (87.2%) belonged to type Ⅳ SCC.
The predominant genotype among MRSA population was ST59-t437-I-IVa (53.8%), followed by ST72-t2431-I-IVF (15.4%). ST72 and CC5 (ST5/965/7197) were the most common hVISA clones. Both CC59 (ST59/7437) and ST72 clones were resistant to erythromycin and clindamycin among MRSA population. Strains of MSSA with phenotypic hVISA (MS-hVISA) exhibited a striking genetic diversity accompanied by the diversification of drug resistance patterns.
调查从黄河三角洲地区患者中分离出的（）的分子流行病学，并构建不同遗传背景下的抗生素耐药谱。
对所有204株菌株进行药敏试验、葡萄球菌蛋白A（）分型和辅助基因调节因子（）分型。对基因阳性和异质性万古霉素中介（hVISA）的分离株进行多位点序列分型（MLST）和葡萄球菌染色体盒式（SCC）分型。
通过基因鉴定出39株耐甲氧西林金黄色葡萄球菌（MRSA）菌株。确认了22株hVISA分离株，包括9株MRSA和13株甲氧西林敏感（MSSA）菌株，其余分离株（n = 182）对万古霉素敏感（VSSA）。94.9%的MRSA和63.6%的hVISA属于I型。57种不同的（）类型包括5种新型，主要为t309（30.9%）、t078（11.8%）和t437（11.8%）。在MRSA和hVISA分离株中，14种序列类型（STs）包括3种新的STs被分为3个克隆复合体（CCs）和7个单倍型。大多数MRSA分离株（87.2%）属于Ⅳ型SCC。
MRSA群体中主要基因型为ST59 - t437 - I - Ⅳa（53.8%），其次是ST72 - t2431 - I - ⅣF（15.4%）。ST72和CC5（ST5/965/7197）是最常见的hVISA克隆。在MRSA群体中，CC59（ST59/7437）和ST72克隆对红霉素和克林霉素均耐药。具有表型hVISA的MSSA菌株（MS - hVISA）表现出显著的遗传多样性，同时伴随着耐药模式的多样化。
