Yao Danyu, Wang Juan, Peng Wentao, Zhang Bowen, Wen Xiaolan, Wan Xiaoneng, Wang Xiuyuan, Li Xinchun, Ma Jian, Liu Xiaofen, Fan Yinglun, Sun Guozhong
National Engineering Laboratory of Crop Molecular Breeding, Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing, China.
College of Agricultural Science and Engineering, Liaocheng University, Liaocheng, Shandong, China.
Front Plant Sci. 2023 Jan 12;13:1099677. doi: 10.3389/fpls.2022.1099677. eCollection 2022.
Low temperature injury in spring has seriously destabilized the production and grain quality of common wheat. However, the molecular mechanisms underlying spring frost tolerance remain elusive. In this study, we investigated the response of a frost-tolerant wheat variety Zhongmai8444 to freezing stress at the meiotic stage. Transcriptome profiles over a time course were subsequently generated by high-throughput sequencing. Our results revealed that the prolonged freezing temperature led to the significant reductions in plant height and seed setting rate. Cell wall thickening in the vascular tissue was also observed in the stems. RNA-seq analyses demonstrated the identification of 1010 up-regulated and 230 down-regulated genes shared by all time points of freezing treatment. Enrichment analysis revealed that gene activity related to hormone signal transduction and cell wall biosynthesis was significantly modulated under freezing. In addition, among the identified differentially expressed genes, 111 transcription factors belonging to multiple gene families exhibited dynamic expression pattern. This study provided valuable gene resources beneficial for the breeding of wheat varieties with improved spring frost tolerance.
春季低温伤害严重破坏了普通小麦的产量和籽粒品质。然而，春性抗冻性的分子机制仍不清楚。在本研究中，我们调查了耐冻小麦品种中麦8444在减数分裂期对冻害胁迫的响应。随后通过高通量测序生成了一个时间进程中的转录组图谱。我们的结果表明，延长的冷冻温度导致株高和结实率显著降低。在茎中还观察到维管组织中的细胞壁增厚。RNA测序分析表明，在冷冻处理的所有时间点共鉴定出1010个上调基因和230个下调基因。富集分析表明，在冷冻条件下，与激素信号转导和细胞壁生物合成相关的基因活性受到显著调节。此外，在鉴定出的差异表达基因中，属于多个基因家族的111个转录因子呈现出动态表达模式。本研究提供了宝贵的基因资源，有助于培育具有更强春性抗冻性的小麦品种。
