Lin Xiao, Torres Ascurra Yerisf Carla, Fillianti Happyka, Dethier Laura, de Rond Laura, Domazakis Emmanouil, Aguilera-Galvez Carolina, Kiros Afewerki Yohannes, Jacobsen Evert, Visser Richard G F, Nürnberger Thorsten, Vleeshouwers Vivianne G A A
Plant Breeding, Wageningen University and Research, Wageningen, Netherlands.
Department of Plant Biochemistry, Centre of Plant Molecular Biology (ZMBP), University of Tübingen, Tübingen, Germany.
Front Plant Sci. 2023 Jan 12;13:1037030. doi: 10.3389/fpls.2022.1037030. eCollection 2022.
Pattern-triggered immunity (PTI) in plants is mediated by cell surface-localized pattern recognition receptors (PRRs) upon perception of microbe-associated molecular pattern (MAMPs). MAMPs are conserved molecules across microbe species, or even kingdoms, and PRRs can confer broad-spectrum disease resistance. Pep-13/25 are well-characterized MAMPs in species, which are renowned devastating oomycete pathogens of potato and other plants, and for which genetic resistance is highly wanted. Pep-13/25 are derived from a 42 kDa transglutaminase GP42, but their cognate PRR has remained unknown. Here, we genetically mapped a novel surface immune receptor that recognizes Pep-25. By using effectoromics screening, we characterized the recognition spectrum of Pep-13/25 in diverse Solanaceae species. Response to Pep-13/25 was predominantly found in potato and related wild tuber-bearing Solanum species. Bulk-segregant RNA sequencing (BSR-Seq) and genetic mapping the response to Pep-25 led to a 0.081 cM region on the top of chromosome 3 in the wild potato species subsp. . Some BAC clones in this region were isolated and sequenced, and we found the Pep-25 receptor locates in a complex receptor-like kinase () locus. This study is an important step toward the identification of the Pep-13/25 receptor, which can potentially lead to broad application in potato and various other hosts of species.
植物中的模式触发免疫（PTI）由细胞表面定位的模式识别受体（PRR）介导，这些受体在感知微生物相关分子模式（MAMP）时发挥作用。MAMP是跨微生物物种甚至跨界的保守分子，PRR可赋予广谱抗病性。Pep-13/25是 物种中特征明确的MAMP， 是马铃薯和其他植物中著名的毁灭性卵菌病原体，人们非常需要对其具有遗传抗性。Pep-13/25源自一种42 kDa的转谷氨酰胺酶GP42，但其同源PRR仍未知。在这里，我们通过遗传定位确定了一种识别Pep-25的新型表面免疫受体。通过效应子组学筛选，我们表征了Pep-13/25在不同茄科物种中的识别谱。对Pep-13/25的反应主要在马铃薯和相关的野生块茎茄属物种中发现。通过混合分离群体RNA测序（BSR-Seq）以及对Pep-25反应的遗传定位，在野生马铃薯物种 亚种的3号染色体顶端确定了一个0.081 cM的区域。分离并测序了该区域的一些BAC克隆，我们发现Pep-25受体位于一个复杂的类受体激酶（ ）基因座中。这项研究是鉴定Pep-13/25受体的重要一步，这可能会在马铃薯和 物种的各种其他宿主中得到广泛应用。
