• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

基于金纳米粒子和还原氧化石墨烯修饰针灸针的 SARS-CoV-2 刺突蛋白分子印迹微型电化学生物传感器。

Molecularly imprinted miniature electrochemical biosensor for SARS-CoV-2 spike protein based on Au nanoparticles and reduced graphene oxide modified acupuncture needle.

机构信息

Jiangsu Key Laboratory of Advanced Catalytic Materials and Technology, School of Petrochemical Engineering, Changzhou University, Changzhou 213164, China.

College of Biological, Chemical Sciences and Engineering, Jiaxing University, Jiaxing 314001, China.

出版信息

Bioelectrochemistry. 2023 Jun;151:108375. doi: 10.1016/j.bioelechem.2023.108375. Epub 2023 Jan 27.

DOI:10.1016/j.bioelechem.2023.108375
PMID:36716516
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9883973/
Abstract

Accurate detection of SARS-CoV-2 spike (SARS-CoV-2-S) protein is of clinical significance for early diagnosis and timely treatment of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Herein, a surface molecularly imprinted miniature biosensor was fabricated. Au nanoparticles (AuNPs), reduced graphene oxide (rGO), poly(methylene blue)/poly(ionic liquids) and poly(ionic liquids) were successively electrodeposited onto the pinpoint of an acupuncture needle (AN). The molecularly imprinted miniature biosensor was obtained after the template of SARS-CoV-2-S protein was removed, which could be used for sensitive detection of SARS-CoV-2-S protein. The linear range and limit of detection (LOD) were 0.1 ∼ 1000 ng mL and 38 pg mL, respectively, which were superior to other molecularly imprinted biosensors previously reported. The developed miniature biosensor also exhibited high specificity and stability. The reliability of the biosensor was evaluated by the detection of SARS-CoV-2-S protein in clinical serum samples.

摘要

准确检测严重急性呼吸综合征冠状病毒 2（SARS-CoV-2）的刺突（SARS-CoV-2-S）蛋白对于早期诊断和及时治疗具有重要的临床意义。在此，构建了一种表面分子印迹微型生物传感器。金纳米粒子（AuNPs）、还原氧化石墨烯（rGO）、聚亚甲基蓝/聚离子液体和聚离子液体依次被电沉积在针灸针的针尖上。去除 SARS-CoV-2-S 蛋白模板后，得到分子印迹微型生物传感器，可用于灵敏检测 SARS-CoV-2-S 蛋白。该传感器的线性范围和检测限（LOD）分别为 0.1~1000ng mL 和 38pg mL，优于之前报道的其他分子印迹生物传感器。所开发的微型生物传感器还表现出高特异性和稳定性。通过检测临床血清样本中的 SARS-CoV-2-S 蛋白来评估生物传感器的可靠性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cf5e/9883973/585997941452/gr8_lrg.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cf5e/9883973/e0927e9e564f/ga1_lrg.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cf5e/9883973/dfe2e49dc34d/gr9_lrg.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cf5e/9883973/ecf35ad504d3/gr1_lrg.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cf5e/9883973/ee298ba7ffba/gr2_lrg.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cf5e/9883973/6d3595d68490/gr3_lrg.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cf5e/9883973/b5873fd9a47b/gr4_lrg.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cf5e/9883973/81e32fe97b13/gr5_lrg.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cf5e/9883973/e0f7c905c519/gr6_lrg.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cf5e/9883973/601bb6e3df3e/gr7_lrg.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cf5e/9883973/585997941452/gr8_lrg.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cf5e/9883973/e0927e9e564f/ga1_lrg.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cf5e/9883973/dfe2e49dc34d/gr9_lrg.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cf5e/9883973/ecf35ad504d3/gr1_lrg.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cf5e/9883973/ee298ba7ffba/gr2_lrg.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cf5e/9883973/6d3595d68490/gr3_lrg.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cf5e/9883973/b5873fd9a47b/gr4_lrg.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cf5e/9883973/81e32fe97b13/gr5_lrg.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cf5e/9883973/e0f7c905c519/gr6_lrg.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cf5e/9883973/601bb6e3df3e/gr7_lrg.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cf5e/9883973/585997941452/gr8_lrg.jpg

相似文献

1
Molecularly imprinted miniature electrochemical biosensor for SARS-CoV-2 spike protein based on Au nanoparticles and reduced graphene oxide modified acupuncture needle.基于金纳米粒子和还原氧化石墨烯修饰针灸针的 SARS-CoV-2 刺突蛋白分子印迹微型电化学生物传感器。
Bioelectrochemistry. 2023 Jun;151:108375. doi: 10.1016/j.bioelechem.2023.108375. Epub 2023 Jan 27.
2
A surface molecularly imprinted electrochemical biosensor for the detection of SARS-CoV-2 spike protein by using CuS-Au as built-in probe.基于 CuS-Au 内置探针的 SARS-CoV-2 刺突蛋白电化学生物传感器的表面分子印迹检测
Bioelectrochemistry. 2023 Aug;152:108462. doi: 10.1016/j.bioelechem.2023.108462. Epub 2023 May 10.
3
Dual-template molecularly imprinted electrochemical biosensor for IgG-IgM combined assay based on a dual-signal strategy.基于双信号策略的用于IgG-IgM联合检测的双模板分子印迹电化学生物传感器。
Bioelectrochemistry. 2022 Dec;148:108267. doi: 10.1016/j.bioelechem.2022.108267. Epub 2022 Sep 16.
4
An ultrasensitive molecularly imprinted polymer-based electrochemical sensor for the determination of SARS-CoV-2-RBD by using macroporous gold screen-printed electrode.基于大孔金丝网印刷电极的 SARS-CoV-2-RBD 分子印迹聚合物电化学传感器的超灵敏测定。
Biosens Bioelectron. 2022 Jan 15;196:113729. doi: 10.1016/j.bios.2021.113729. Epub 2021 Oct 26.
5
Synthesis of gold nanoparticles@reduced porous graphene-modified ITO electrode for spectroelectrochemical detection of SARS-CoV-2 spike protein.金纳米粒子@还原多孔石墨烯修饰 ITO 电极的合成及其用于 SARS-CoV-2 刺突蛋白的光谱电化学检测。
Spectrochim Acta A Mol Biomol Spectrosc. 2022 Jan 5;264:120237. doi: 10.1016/j.saa.2021.120237. Epub 2021 Jul 28.
6
Label-free detection of SARS-CoV-2 Spike S1 antigen triggered by electroactive gold nanoparticles on antibody coated fluorine-doped tin oxide (FTO) electrode.基于抗体修饰氟掺杂氧化锡（FTO）电极上的电化学生物传感器检测 SARS-CoV-2 Spike S1 抗原
Anal Chim Acta. 2021 Dec 15;1188:339207. doi: 10.1016/j.aca.2021.339207. Epub 2021 Oct 21.
7
Development and characterisation of cysteine-based gold electrodes for the electrochemical biosensing of the SARS-CoV-2 spike antigen.基于半胱氨酸的金电极的开发与特性及其在 SARS-CoV-2 刺突抗原电化学生物传感中的应用。
Analyst. 2022 Oct 10;147(20):4462-4472. doi: 10.1039/d2an01225a.
8
Molecularly imprinted composite-based biosensor for the determination of SARS-CoV-2 nucleocapsid protein.基于分子印迹复合材料的用于测定 SARS-CoV-2 核衣壳蛋白的生物传感器。
Biosens Bioelectron. 2024 May 1;251:116043. doi: 10.1016/j.bios.2024.116043. Epub 2024 Feb 5.
9
Electrochemical Sensing of α-Fetoprotein Based on Molecularly Imprinted Polymerized Ionic Liquid Film on a Gold Nanoparticle Modified Electrode Surface.基于金纳米粒子修饰电极表面分子印迹聚合离子液体膜的电化学传感α-胎蛋白
Sensors (Basel). 2019 Jul 22;19(14):3218. doi: 10.3390/s19143218.
10
An electrochemical chlorpromazine sensor based on a gold-copper bimetallic synergetic molecularly imprinted interface on an acupuncture needle electrode.一种基于针灸针电极上金-铜双金属协同分子印迹界面的电化学氯丙嗪传感器。
Analyst. 2023 May 16;148(10):2214-2224. doi: 10.1039/d3an00373f.

引用本文的文献

1
Comparative Roles of Hydrogels, Deep Eutectic Solvents, and Ionic Liquids in Enzyme-Based Biosensors, Bioelectronics and Biomimetics Devices.水凝胶、低共熔溶剂和离子液体在基于酶的生物传感器、生物电子学和仿生器件中的比较作用
ACS Meas Sci Au. 2025 Jul 15;5(4):424-442. doi: 10.1021/acsmeasuresciau.5c00036. eCollection 2025 Aug 20.
2
Needle-Shaped Biosensors for Precision Diagnoses: From Benchtop Development to In Vitro and In Vivo Applications.用于精准诊断的针状生物传感器：从台式研发到体外和体内应用。
Biosensors (Basel). 2024 Aug 13;14(8):391. doi: 10.3390/bios14080391.
3
Hierarchical Nanobiosensors at the End of the SARS-CoV-2 Pandemic.

本文引用的文献

1
An electrochemical biosensor for SARS-CoV-2 detection via its papain-like cysteine protease and the protease inhibitor screening.一种通过SARS-CoV-2的木瓜蛋白酶样半胱氨酸蛋白酶进行检测及蛋白酶抑制剂筛选的电化学生物传感器。
Chem Eng J. 2023 Jan 15;452:139646. doi: 10.1016/j.cej.2022.139646. Epub 2022 Oct 10.
2
Label-free electrochemical aptasensor for the detection of SARS-CoV-2 spike protein based on carbon cloth sputtered gold nanoparticles.基于碳布溅射金纳米颗粒的无标记电化学适体传感器用于检测新型冠状病毒刺突蛋白
Biosens Bioelectron X. 2022 Dec;12:100256. doi: 10.1016/j.biosx.2022.100256. Epub 2022 Sep 24.
3
Dual-template molecularly imprinted electrochemical biosensor for IgG-IgM combined assay based on a dual-signal strategy.
新冠疫情末期的层级式纳米生物传感器。
Biosensors (Basel). 2024 Feb 18;14(2):108. doi: 10.3390/bios14020108.
基于双信号策略的用于IgG-IgM联合检测的双模板分子印迹电化学生物传感器。
Bioelectrochemistry. 2022 Dec;148:108267. doi: 10.1016/j.bioelechem.2022.108267. Epub 2022 Sep 16.
4
An ultra-sensitive and specific nanoplasmonic-enhanced isothermal amplification platform for the ultrafast point-of-care testing of SARS-CoV-2.一种用于SARS-CoV-2超快速即时检测的超灵敏、高特异性的纳米等离子体增强等温扩增平台。
Chem Eng J. 2023 Jan 1;451:138822. doi: 10.1016/j.cej.2022.138822. Epub 2022 Aug 28.
5
Sensitive and reproducible detection of SARS-CoV-2 using SERS-based microdroplet sensor.使用基于表面增强拉曼光谱（SERS）的微滴传感器灵敏且可重复地检测严重急性呼吸综合征冠状病毒2（SARS-CoV-2）
Chem Eng J. 2022 Oct 15;446:137085. doi: 10.1016/j.cej.2022.137085. Epub 2022 May 20.
6
Antifouling ionic liquid doped molecularly imprinted polymer-based ratiometric electrochemical sensor for highly stable and selective detection of zearalenone.基于离子液体掺杂分子印迹聚合物的比率型电化学传感器用于玉米赤霉烯酮的高稳定性和选择性检测。
Anal Chim Acta. 2022 Jun 1;1210:339884. doi: 10.1016/j.aca.2022.339884. Epub 2022 Apr 30.
7
Population antibody responses following COVID-19 vaccination in 212,102 individuals.212102 例个体接种 COVID-19 疫苗后的人群抗体反应。
Nat Commun. 2022 Feb 16;13(1):907. doi: 10.1038/s41467-022-28527-x.
8
A surface protein-imprinted biosensor based on boronate affinity for the detection of anti-human immunoglobulin G.基于硼酸亲和作用的表面蛋白印迹生物传感器用于检测抗人免疫球蛋白 G。
Mikrochim Acta. 2022 Feb 15;189(3):106. doi: 10.1007/s00604-022-05204-w.
9
Molecularly imprinted polypyrrole based sensor for the detection of SARS-CoV-2 spike glycoprotein.用于检测新型冠状病毒刺突糖蛋白的基于分子印迹聚吡咯的传感器。
Electrochim Acta. 2022 Jan 20;403:139581. doi: 10.1016/j.electacta.2021.139581. Epub 2021 Nov 16.
10
Molecularly imprinted polymer based electrochemical sensor for quantitative detection of SARS-CoV-2 spike protein.基于分子印迹聚合物的电化学传感器用于定量检测新型冠状病毒刺突蛋白
Sens Actuators B Chem. 2022 Feb 15;353:131160. doi: 10.1016/j.snb.2021.131160. Epub 2021 Nov 27.