微小RNA-149通过抑制血管细胞黏附分子-1和使PI3K/AKT信号通路失活来改善骨关节炎。
MicroRNA-149 improves osteoarthritis via repression of VCAM-1 and inactivation of PI3K/AKT pathway.
作者信息
Jiang Yongqin, Zhang Lei, Tian Huiyu
机构信息
Department of Orthopedics, The Second Affiliated Hospital of Harbin Medical University, Harbin 150086, Heilongjiang, China.
Department of Physical Diagnosis, The Second Hospital of Heilongjiang Province, Harbin 150001, Heilongjiang, China.
出版信息
Exp Gerontol. 2023 Apr;174:112103. doi: 10.1016/j.exger.2023.112103. Epub 2023 Jan 27.
OBJECTIVE
MicroRNAs (miRNAs) are key regulators in osteoarthritis (OA). While the role of miR-149 in OA has not been fully understood yet. This study investigated the mechanism in which miR-149 inhibited vascular cell adhesion molecule 1 (VCAM-1) via depressing PI3K/AKT pathway, thereby alleviating OA.
METHODS
A mouse OA model was constructed. The mice were injected with miR-149, VCAM-1- PI3K/AKT pathway-related sequences to figure their roles in OA. Inflammation and apoptosis were detected in the cartilage tissues of mice. MiR-149 and VCAM-1expression were detected.
RESULTS
Decreased miR-149 and enhanced VCAM-1 existed in cartilage tissues of patients with OA. Elevated miR-149 or suppressed VCAM-1 limited inflammation and apoptosis in cartilage tissues of mice with OA, which was related to PI3K/AKT pathway inactivation.
CONCLUSION
Our study provides evidence that up-regulated miR-149 alleviates OA via inhibition of VCAM-1 and PI3K/AKT pathway, which is helpful for OA treatment.
目的
微小RNA（miRNA）是骨关节炎（OA）的关键调节因子。然而，miR-149在OA中的作用尚未完全明确。本研究探讨miR-149通过抑制PI3K/AKT信号通路抑制血管细胞黏附分子1（VCAM-1），从而减轻OA的机制。
方法
构建小鼠OA模型。给小鼠注射miR-149、VCAM-1及PI3K/AKT信号通路相关序列，以明确它们在OA中的作用。检测小鼠软骨组织中的炎症和凋亡情况。检测miR-149和VCAM-1的表达。
结果
OA患者软骨组织中miR-149表达降低，VCAM-1表达升高。上调miR-149或抑制VCAM-1可减轻OA小鼠软骨组织的炎症和凋亡，这与PI3K/AKT信号通路失活有关。
结论
我们的研究表明，上调miR-149通过抑制VCAM-1和PI3K/AKT信号通路减轻OA，这对OA治疗有帮助。
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