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抑制 miR-199b-5p 可能通过靶向 和 减少骨关节炎的病理改变。

Inhibition of miR-199b-5p reduces pathological alterations in osteoarthritis by potentially targeting and .

机构信息

Acupuncture and Tuina College, Chengdu University of Traditional Chinese Medicine, Chengdu, China.

Chongqing Hospital of Traditional Chinese Medicine, Chongqing, China.

出版信息

Elife. 2024 May 21;12:RP92645. doi: 10.7554/eLife.92645.

DOI:10.7554/eLife.92645
PMID:38770735
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11108644/
Abstract

Osteoarthritis (OA) is a degenerative disease with a high prevalence in the elderly population, but our understanding of its mechanisms remains incomplete. Analysis of serum exosomal small RNA sequencing data from clinical patients and gene expression data from OA patient serum and cartilage obtained from the GEO database revealed a common dysregulated miRNA, miR-199b-5p. In vitro cell experiments demonstrated that miR-199b-5p inhibits chondrocyte vitality and promotes extracellular matrix degradation. Conversely, inhibition of miR-199b-5p under inflammatory conditions exhibited protective effects against damage. Local viral injection of miR-199b-5p into mice induced a decrease in pain threshold and OA-like changes. In an OA model, inhibition of miR-199b-5p alleviated the pathological progression of OA. Furthermore, bioinformatics analysis and experimental validation identified and as potential target genes of . Thus, these results indicated that / and axis might be a novel therapeutic target for the treatment of OA.

摘要

骨关节炎（OA）是一种退行性疾病，在老年人群中发病率较高，但我们对其发病机制的理解仍不完整。对临床患者血清外泌体小 RNA 测序数据和从 GEO 数据库获得的 OA 患者血清和软骨基因表达数据进行分析，发现了一个常见的失调 miRNA，miR-199b-5p。体外细胞实验表明，miR-199b-5p 抑制软骨细胞活力并促进细胞外基质降解。相反，在炎症条件下抑制 miR-199b-5p 对损伤具有保护作用。将 miR-199b-5p 局部病毒注射到小鼠体内会导致疼痛阈值降低和类似 OA 的变化。在 OA 模型中，抑制 miR-199b-5p 可减轻 OA 的病理进展。此外，生物信息学分析和实验验证确定 和 是 的潜在靶基因。因此，这些结果表明 / 和 轴可能是治疗 OA 的新的治疗靶点。

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