匙羹藤提取物在抑制糖尿病视网膜病变进展中的疗效：一项研究。
Efficacy of Sambiloto Extracts, in Inhibiting Diabetic Retinopathy Progression: An Study.
作者信息
Amin Ramzi, Shariff Muhammad Apriliandy, Purwanita Petty, Saleh Mgs Irsan
机构信息
Department of Ophthalmology, Faculty of Medicine, Universitas Sriwijaya/Dr Moh Hoesin General Hospital, Palembang, Indonesia.
Specialized Residency Training, Faculty of Medicine, Universitas Sriwijaya/Dr Moh Hoesin General Hospital, Palembang, Indonesia.
出版信息
Rep Biochem Mol Biol. 2022 Oct;11(3):457-464. doi: 10.52547/rbmb.11.3.457.
BACKGROUND
Diabetic retinopathy (DR) is one of diabetes mellitus complication and occurred in retinal microvascular. This study was aimed to investigate the efficacy of Sambiloto, extract on glycemic profile, antioxidant and inflammatory cytokine parameters in diabetic rats, and phytochemical analysis of .
METHODS
extract (APE) was carried out by maceration with ethanol. Diabetes mellitus in Wistar male rats was induced with streptozotocin. Retinal vessel diameters were estimated using a method by Vucetic. Inflammatory cytokine and antioxidant parameters were evaluated in retinal tissue. The alkaloid and flavonoid contents in extract were analyzed using thin layer chromatography method.
RESULTS
Funduscopic examination presented some changes in the diameter of the blood vessels. The vessel diameter in the diabetic retinopathy group with APE in concentration of 100 and 200 mg/kg BW groups was significantly lower than in the DR group (p<0.05). The administration of APE in dosages of 100 and 200 mg/kg BW showed reduced glutathione, SOD, and catalase levels compared to the DR group (p<0.05).
CONCLUSION
extract doses of 100 and 200 mg/kg BW improved diabetic retinopathy in rats through hypoglycemic effects, antioxidant effects, and anti-inflammatory mechanisms.
背景
糖尿病视网膜病变（DR）是糖尿病的并发症之一，发生于视网膜微血管。本研究旨在探讨 Sambiloto 提取物对糖尿病大鼠血糖水平、抗氧化和炎症细胞因子参数的影响，以及对其进行植物化学分析。
方法
采用乙醇浸渍法制备提取物（APE）。用链脲佐菌素诱导雄性 Wistar 大鼠患糖尿病。采用 Vucetic 法估计视网膜血管直径。评估视网膜组织中的炎症细胞因子和抗氧化参数。采用薄层色谱法分析提取物中的生物碱和黄酮含量。
结果
眼底检查显示血管直径有一些变化。糖尿病视网膜病变组中，APE 浓度为 100 和 200 mg/kg 体重组的血管直径显著低于 DR 组（p<0.05）。与 DR 组相比，给予 100 和 200 mg/kg 体重剂量的 APE 后，谷胱甘肽、超氧化物歧化酶和过氧化氢酶水平降低（p<0.05）。
结论
100 和 200 mg/kg 体重剂量的提取物通过降血糖作用、抗氧化作用和抗炎机制改善了大鼠的糖尿病视网膜病变。
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