Extended-spectrum beta-lactamase-producing from pork in Muang district, Chiang Mai Province, Thailand.

BACKGROUND AND AIM

Contaminated pork is one of the transmission routes for pathogens. Extended-spectrum beta-lactamases (ESBL)-producing is one of the critical threats to global public health. This study aimed to examine pork from different types of markets in Muang district, Chiangmai Province, Thailand, for a proportion of ESBL-producing , antibiotic resistance of ESBL-producing and ESBL-producing genotypes.

MATERIALS AND METHODS

Samples were collected from different market types; fresh markets, pork stores, and supermarkets, enriched and inoculated on selective media. Extended-spectrum beta-lactamases-producing was identified using double-disk diffusion method according to Clinical and Laboratory Standards Institute 2016. Antibiotic susceptibility test was performed through VITEK System and ESBL-encoding genes were detected using a multiplex polymerase chain reaction.

RESULTS

About 69% of the samples were positive to ESBL-producing and showed high rates of resistance for ampicillin (100%), piperacillin (100%), cefalexin (100%), cefpodoxime (100%), cefovecin (100%) and ceftiofur (100%), gentamycin (89.86%), and tetracycline (TE) (84.06%). All isolates were multiple drug resistant; resistance patterns of beta-lactams, aminoglycosides, TEs, nitrobenzene derivatives, and sulfonamide groups were observed. The ESBL-producing positive isolates carried CTX-M groups (100%), TEM (98.55%), and SHV (1.45%). None of OXA was found in this study.

CONCLUSION

Extended-spectrum beta-lactamases-producing was found in various types of markets; all isolates were detected as multidrug-resistant. The dissemination of such strains can conceivably cause concerning public health, implying that supervised antimicrobial use in pork production and sanitary food preparation is recommended.