Srichumporn Wirunchana, Chaisowwong Warangkhana, Intanon Montira, Na-Lampang Kannika
Faculty of Veterinary Medicine, Chiang Mai University, Chiang Mai, 50100, Thailand.
Department of Veterinary Bioscience and Public Health, Faculty of Veterinary Medicine, Chiang Mai University, Chiang Mai, 50100, Thailand.
Vet World. 2022 Dec;15(12):2903-2909. doi: 10.14202/vetworld.2022.2903-2909. Epub 2022 Dec 21.
Contaminated pork is one of the transmission routes for pathogens. Extended-spectrum beta-lactamases (ESBL)-producing is one of the critical threats to global public health. This study aimed to examine pork from different types of markets in Muang district, Chiangmai Province, Thailand, for a proportion of ESBL-producing , antibiotic resistance of ESBL-producing and ESBL-producing genotypes.
Samples were collected from different market types; fresh markets, pork stores, and supermarkets, enriched and inoculated on selective media. Extended-spectrum beta-lactamases-producing was identified using double-disk diffusion method according to Clinical and Laboratory Standards Institute 2016. Antibiotic susceptibility test was performed through VITEK System and ESBL-encoding genes were detected using a multiplex polymerase chain reaction.
About 69% of the samples were positive to ESBL-producing and showed high rates of resistance for ampicillin (100%), piperacillin (100%), cefalexin (100%), cefpodoxime (100%), cefovecin (100%) and ceftiofur (100%), gentamycin (89.86%), and tetracycline (TE) (84.06%). All isolates were multiple drug resistant; resistance patterns of beta-lactams, aminoglycosides, TEs, nitrobenzene derivatives, and sulfonamide groups were observed. The ESBL-producing positive isolates carried CTX-M groups (100%), TEM (98.55%), and SHV (1.45%). None of OXA was found in this study.
Extended-spectrum beta-lactamases-producing was found in various types of markets; all isolates were detected as multidrug-resistant. The dissemination of such strains can conceivably cause concerning public health, implying that supervised antimicrobial use in pork production and sanitary food preparation is recommended.
受污染猪肉是病原体的传播途径之一。产超广谱β-内酰胺酶（ESBL）的细菌是全球公共卫生面临的重大威胁之一。本研究旨在检测泰国清迈府孟县不同类型市场的猪肉中产ESBL细菌的比例、产ESBL细菌的抗生素耐药性及产ESBL细菌的基因型。
从不同类型市场采集样本，包括生鲜市场、猪肉店和超市，进行富集并接种于选择性培养基上。根据临床和实验室标准协会2016年标准，采用双纸片扩散法鉴定产超广谱β-内酰胺酶细菌。通过VITEK系统进行抗生素敏感性试验，并使用多重聚合酶链反应检测ESBL编码基因。
约69%的样本对产ESBL细菌呈阳性，且对氨苄西林（100%）、哌拉西林（100%）、头孢氨苄（100%）、头孢泊肟（100%）、头孢维星（100%）、头孢噻呋（100%）、庆大霉素（89.86%）和四环素（84.06%）显示出高耐药率。所有分离株均具有多重耐药性，观察到β-内酰胺类、氨基糖苷类、四环素类、硝基苯衍生物和磺胺类的耐药模式。产ESBL阳性分离株携带CTX-M型（100%）、TEM型（98.55%）和SHV型（1.45%）。本研究未发现OXA型。
在各类市场均发现了产超广谱β-内酰胺酶细菌；所有分离株均检测为多重耐药。此类菌株的传播可能会引发公共卫生问题，这意味着建议在猪肉生产和卫生食品制备过程中监督抗菌药物的使用。
