Aini Eka Puji Dameanti Fidi Nur, Yanestria Sheila Marty, Effendi Mustofa Helmi, Plumeriastuti Hani, Tyasningsih Wiwiek, Ugbo Emmanuel Nnabuike, Sutrisno Rahayu, Safri Muhammad Ali Akramsyah
Laboratory of Microbiology and Immunology Veterinary, Faculty of Veterinary Medicine, Universitas Brawijaya. Jl. Puncak Dieng, Kalisongo, Malang Regency, 65151. East Java, Indonesia.
Department of Veterinary Public Health, Wijaya Kusuma Surabaya University, Jl. Dukuh Kupang XXV No.54, Dukuh Kupang, Dukuhpakis, Surabaya, 60225, East Java, Indonesia.
Vet World. 2025 Jan;18(1):162-171. doi: 10.14202/vetworld.2025.162-171. Epub 2025 Jan 27.
Extended-spectrum beta-lactamase (ESBL)-producing in dairy farm wastewater represents a significant threat to environmental and public health due to the dissemination of antibiotic-resistance genes. This study investigates the molecular profiles of ESBL-producing isolates harboring , , and genes from dairy farm wastewater in East Java, Indonesia.
Wastewater samples (n = 342) were collected from six major dairy regions in East Java: Pasuruan, Malang, Tulungagung, Blitar, Batu, and Kediri. The samples underwent bacterial isolation, identification, and ESBL phenotypic screening using the double-disk synergy test (DDST). Molecular genotyping of , , and was conducted using a polymerase chain reaction.
Of the samples, 69.30% (237/342) tested positive for , with 32.91% (78/237) identified as ESBL producers through DDST. Molecular genotyping confirmed ESBL genes in 28.20% (22/78) of the isolates. The genotypic distribution was 77.2% (17/22) , 4.5% (1/22) , 9.0% (2/22) + , and 9.0% (2/22) + . These findings highlight the dominance of and the presence of multi-gene combinations in East Java's dairy farm wastewater.
The identified molecular profiles underscore dairy farm wastewater as a critical reservoir for antibiotic resistance genes, particularly . Addressing this issue necessitates stringent antimicrobial use policies, improved wastewater management, and enhanced biosecurity measures. These findings support a One Health approach to mitigate environmental and public health risks associated with AMR.
由于抗生素抗性基因的传播，奶牛场废水中产生超广谱β-内酰胺酶（ESBL）的细菌对环境和公众健康构成重大威胁。本研究调查了印度尼西亚东爪哇奶牛场废水中携带blaCTX-M、blaSHV和blaTEM基因的产ESBL菌株的分子特征。
从东爪哇的六个主要奶牛养殖区收集废水样本（n = 342）：巴苏鲁安、玛琅、图龙阿贡、勿里达、巴图和凯迪里。样本经过细菌分离、鉴定，并使用双纸片协同试验（DDST）进行ESBL表型筛选。使用聚合酶链反应对blaCTX-M、blaSHV和blaTEM进行分子基因分型。
在样本中，69.30%（237/342）的样本对blaTEM检测呈阳性，其中32.91%（78/237）通过DDST鉴定为产ESBL菌株。分子基因分型在28.20%（22/78）的分离株中确认了ESBL基因。基因型分布为77.2%（17/22）blaCTX-M、4.5%（1/22）blaSHV、9.0%（2/22）blaCTX-M + blaSHV和9.0%（2/22）blaCTX-M + blaTEM。这些发现突出了blaCTX-M在东爪哇奶牛场废水中的优势地位以及多基因组合的存在。
所确定的分子特征强调了奶牛场废水是抗生素抗性基因，特别是blaCTX-M的关键储存库。解决这一问题需要严格的抗菌药物使用政策、改善废水管理以及加强生物安全措施。这些发现支持采用“同一健康”方法来减轻与抗菌药物耐药性相关的环境和公共卫生风险。
