Mougios Nikolaos, Opazo Felipe, Rizzoli Silvio O, Reshetniak Sofiia
Institute of Neuro- and Sensory Physiology, University Medical Center Göttingen, 37073 Göttingen, Germany.
Center for Biostructural Imaging of Neurodegeneration (BIN), University of Göttingen Medical Center, 37075 Göttingen, Germany.
iScience. 2023 Jan 13;26(2):105971. doi: 10.1016/j.isci.2023.105971. eCollection 2023 Feb 17.
The function of the postsynaptic compartment is based on the presence and activity of postsynaptic receptors, whose dynamics are controlled by numerous scaffolding, signaling and trafficking proteins. Although the receptors and the scaffolding proteins have received substantial attention, the trafficking proteins have not been investigated extensively. Their mobility rates are unknown, and it is unclear how the postsynaptic environment affects their dynamics. To address this, we analyzed several trafficking proteins (α-synuclein, amphiphysin, calmodulin, doc2a, dynamin, and endophilin), estimating their movement rates in the dendritic shaft, as well as in morphologically distinct "mushroom" and "stubby" postsynapse types. The diffusion parameters were surprisingly similar across dendritic compartments, and a few differences between proteins became evident only in the presence of a synapse neck. We conclude that the movement of trafficking proteins is not strongly affected by the postsynaptic compartment, in stark contrast to the presynapse, which regulates strongly the movement of such proteins.
突触后区室的功能基于突触后受体的存在和活性，其动态受众多支架、信号和运输蛋白的控制。尽管受体和支架蛋白已受到大量关注，但运输蛋白尚未得到广泛研究。它们的移动速率未知，并且尚不清楚突触后环境如何影响其动态。为了解决这个问题，我们分析了几种运输蛋白（α-突触核蛋白、发动蛋白、钙调蛋白、doc2a、动力蛋白和内吞蛋白），估计它们在树突干以及形态上不同的“蘑菇”和“短粗”突触后类型中的移动速率。跨树突区室的扩散参数惊人地相似，只有在存在突触颈的情况下，蛋白质之间的一些差异才变得明显。我们得出结论，运输蛋白的移动不受突触后区室的强烈影响，这与对这类蛋白移动有强烈调节作用的突触前区室形成鲜明对比。
