Duan Xiaojie, Leng Yujia, Chen Fusheng, Zhang Min, Li Zihui
College of Food Science and Technology, Henan University of Technology, Zhengzhou, 450001, Henan, China.
Amino Acids. 2023 Mar;55(3):359-370. doi: 10.1007/s00726-023-03234-z. Epub 2023 Jan 31.
In this study, the potential of oilseed proteins from soybean, peanut, sesame, sunflower seed and flaxseed as antimicrobial peptide (AMP) precursors was assessed using the bioinformatics method. Thirty-four novel potential AMPs were obtained by in silico hydrolysis of 12 oilseed protein sequences, and 11 of them were positive in all four algorithm tests in CAMP. Among the six proteases analyzed, trypsin cleaved soybean, peanut, sesame and sunflower seed proteins most effectively to generate AMPs, with three, four, two and two AMPs obtained, respectively. Subtilisin was most effective for flaxseed AMPs release, obtaining three AMPs. More than 85% of AMPs were predicted to be cationic peptides, and some AMPs were hydrophobic. These potential AMPs were classified as non-toxic peptides, and 15 peptides were non-allergenic. All the AMPs were unstable to digestive enzymes according to in silico simulated digestion. The results of this study provide a theoretical basis for further development of AMPs using oilseed proteins.
在本研究中，采用生物信息学方法评估了大豆、花生、芝麻、向日葵籽和亚麻籽的油籽蛋白作为抗菌肽（AMP）前体的潜力。通过对12个油籽蛋白序列进行计算机模拟水解，获得了34种新型潜在抗菌肽，其中11种在CAMP的所有四种算法测试中均呈阳性。在所分析的六种蛋白酶中，胰蛋白酶对大豆、花生、芝麻和向日葵籽蛋白的切割效果最有效，分别获得了三种、四种、两种和两种抗菌肽。枯草杆菌蛋白酶对亚麻籽抗菌肽的释放最有效，获得了三种抗菌肽。超过85%的抗菌肽被预测为阳离子肽，一些抗菌肽具有疏水性。这些潜在抗菌肽被归类为无毒肽，15种肽无致敏性。根据计算机模拟消化结果，所有抗菌肽对消化酶均不稳定。本研究结果为利用油籽蛋白进一步开发抗菌肽提供了理论依据。
