Department of Zoology and Entomology, Faculty of Science, Assiut University, Asyût, 71516, Egypt.
Sci Rep. 2023 Jan 31;13(1):1732. doi: 10.1038/s41598-023-28860-1.
This study aimed to determine the effect of gallic acid (GA) on ameliorating bisphenol A (BPA) nephrotoxicity in male rat kidneys. Forty rats were assigned randomly into two groups: control (ten animals) and BPA (40 mg/kg bwt) (thirty animals), the second group was divided into three subgroups: BPA alone, BPA + G50 (50 mg/kg bwt), and BPA + G200 (200 mg/kg bwt). The biochemical analysis included measurements of the contents of nitric oxide, lipid peroxidation, reactive oxygen species, and cytokines (interleukin-1α and interleukin-6) in the kidney. The antioxidant enzymes catalase and superoxide dismutase were also measured in the kidney. Kidney function was assessed by determining uric acid, urea, and creatinine levels. The morphological investigations included hematoxylin and eosin staining for assessing the general histology and determining the glomerular and corpuscular areas, the tubular cell degeneration mean area, and the mean leukocyte infiltration area. Also, collagen fiber intensity and polysaccharide content were analyzed. Furthermore, immunohistochemical, morphometric, and ultrastructural studies were carried out. The results revealed morphological, immunohistochemical, and biochemical alterations in the kidney. Most of these changes showed a satisfactory improvement of kidney damage when BPA-administered rats were treated with GA at both doses. In conclusion, GA exhibited a strong protective effect against BPA-induced nephrotoxicity.
本研究旨在确定没食子酸（GA）对改善雄性大鼠肾脏中双酚 A（BPA）肾毒性的作用。将 40 只大鼠随机分为两组：对照组（10 只动物）和 BPA（40mg/kg bwt）组（30 只动物），第二组分为三组：单独 BPA 组、BPA+G50（50mg/kg bwt）组和 BPA+G200（200mg/kg bwt）组。生化分析包括测量肾脏中一氧化氮、脂质过氧化、活性氧和细胞因子（白细胞介素-1α和白细胞介素-6）的含量。还测量了肾脏中的抗氧化酶过氧化氢酶和超氧化物歧化酶。通过测定尿酸、尿素和肌酐水平来评估肾功能。形态学研究包括苏木精和伊红染色，以评估一般组织学并确定肾小球和胞体面积、肾小管细胞变性平均面积和白细胞浸润平均面积。还分析了胶原纤维强度和多糖含量。此外，进行了免疫组织化学、形态计量学和超微结构研究。结果显示肾脏发生了形态、免疫组织化学和生化改变。当给予两种剂量 GA 治疗时，BPA 给药大鼠的大多数这些变化显示出对肾脏损伤的改善令人满意。总之，GA 对 BPA 诱导的肾毒性表现出很强的保护作用。
