Affiliated Stomatology Hospital of Guangzhou Medical University, Guangdong Engineering Research Center of Oral Restoration and Reconstruction, Guangzhou Key Laboratory of Basic and Applied Research of Oral Regenerative Medicine, Guangzhou, China.
Laboratory for Myology, Department of Human Movement Sciences, Faculty of Behavioural and Movement Sciences, Vrije Universiteit Amsterdam, Amsterdam Movement Sciences, Amsterdam, The Netherlands.
J Cell Mol Med. 2023 Feb;27(4):515-528. doi: 10.1111/jcmm.17672. Epub 2023 Feb 1.
Due to the lack of effective treatments, osteoarthritis (OA) remains a challenge for clinicians. Quercetin, a bioflavonoid, has shown potent anti-inflammatory effects. However, its effect on preventing OA progression and the underlying mechanisms are still unclear. In this study, Sprague-Dawley male rats were divided into five groups: control group, OA group (monosodium iodoacetate intra-articular injection), and three quercetin-treated groups. Quercetin-treated groups were treated with intragastric quercetin once a day for 28 days. Gross observation and histopathological analysis showed cartilage degradation and matrix loss in the OA group. High-dose quercetin-group joints showed failure in OA progression. High-dose quercetin inhibited the OA-induced expression of MMP-3, MMP-13, ADAMTS4, and ADAMTS5 and promoted the OA-reduced expression of aggrecan and collagen II. Levels of most inflammatory cytokines and growth factors tested in synovial fluid and serum were upregulated in the OA group and these increases were reversed by high-dose quercetin. Similarly, subchondral trabecular bone was degraded in the OA group and this effect was reversed in the high-dose quercetin group. Our findings indicate that quercetin has a protective effect against OA development and progression possibly via maintaining the inflammatory cascade homeostasis. Therefore, quercetin could be a potential therapeutic agent to prevent OA progression in risk groups.
由于缺乏有效的治疗方法，骨关节炎（OA）仍然是临床医生面临的挑战。槲皮素是一种生物类黄酮，具有很强的抗炎作用。然而，其预防 OA 进展的作用及其潜在机制尚不清楚。在这项研究中，将 Sprague-Dawley 雄性大鼠分为五组：对照组、OA 组（关节内注射单碘乙酸钠）和三个槲皮素治疗组。槲皮素治疗组每天通过灌胃给予槲皮素一次，共 28 天。大体观察和组织病理学分析显示 OA 组软骨降解和基质丢失。高剂量槲皮素组关节 OA 进展失败。高剂量槲皮素抑制 OA 诱导的 MMP-3、MMP-13、ADAMTS4 和 ADAMTS5 的表达，并促进 OA 降低的聚集蛋白聚糖和胶原 II 的表达。OA 组关节滑液和血清中大多数炎性细胞因子和生长因子的水平升高，高剂量槲皮素逆转了这些升高。同样，OA 组的软骨下小梁骨降解，高剂量槲皮素组则逆转了这一效应。我们的研究结果表明，槲皮素对 OA 的发展和进展具有保护作用，可能是通过维持炎症级联的内稳态。因此，槲皮素可能是预防高危人群 OA 进展的潜在治疗药物。
