Department of Nanotechnology, SRM Institute of Science and Technology, Kattankulathur603203, Chengalpattu District, Tamil Nadu, India.
Translational Health Science and Technology Institute, Faridabad121001, Haryana, India.
ACS Appl Bio Mater. 2023 Feb 20;6(2):615-627. doi: 10.1021/acsabm.2c00903. Epub 2023 Feb 1.
Wounds are a serious life threat that occurs in daily life. The complex cascade of synchronized cellular and molecular phases in wound healing is impaired by different means, involving infection, neuropathic complexes, abnormal blood circulation, and cell proliferation at the wound region. Thus, to overcome these problems, a multifunctional wound dressing material is fabricated. In the current research work, we have fabricated a wound dressing polymeric patch, with poly(vinyl alcohol) (PVA) and chitosan (Cs) incorporated with a photocatalytic graphene nanocomposite (GO/TiO(V-N)) and curcumin by a gel casting method, that focuses on multiple stages of the healing process. The morphology, swelling, degradation, moisture vapor transmission rate (MVTR), porosity, light-induced antibacterial activity, hemolysis, blood clotting, blood abortion, light-induced biocompatibility, migration assay, and drug release were analyzed for the polymeric patches under in vitro conditions. PVA/Cs/GO/TiO(V-N)/Cur patches have shown enhanced wound healing in in vivo wound healing experiments on Wister rats. They show higher collagen deposition, thicker granulation tissue, and higher fibroblast density than conventional dressing. A histological study shows excellent re-epithelialization ability and dense collagen deposition. In vitro and in vivo analysis confirmed that PVA/Cs/GO/TiO(V-N) and PVA/Cs/GO/TiO(V-N)/Cur patches enhance the wound healing process.
伤口是日常生活中严重的生命威胁。伤口愈合过程中复杂的细胞和分子级联同步受到多种方式的损害，包括感染、神经病理性复合物、血液循环异常和伤口区域的细胞增殖。因此，为了克服这些问题，制备了一种多功能伤口敷料材料。在当前的研究工作中，我们通过凝胶浇铸法制备了一种含有光催化石墨烯纳米复合材料（GO/TiO(V-N)）和姜黄素的聚（乙烯醇）（PVA）和壳聚糖（Cs）的伤口敷料聚合物贴片，重点关注愈合过程的多个阶段。对聚合物贴片进行了形态学、溶胀、降解、湿气传输速率（MVTR）、孔隙率、光诱导抗菌活性、溶血、凝血、血液流产、光诱导生物相容性、迁移试验和药物释放分析。在 Wister 大鼠的体内伤口愈合实验中，PVA/Cs/GO/TiO(V-N)/Cur 贴片显示出增强的伤口愈合效果。它们显示出比传统敷料更高的胶原沉积、更厚的肉芽组织和更高的成纤维细胞密度。组织学研究显示出出色的再上皮化能力和密集的胶原沉积。体外和体内分析证实，PVA/Cs/GO/TiO(V-N)和 PVA/Cs/GO/TiO(V-N)/Cur 贴片可促进伤口愈合过程。
