Ding Tongbin, Gao Zhe, Liu Hang, Li Yuan, Liu Shichao, Jian Liguo
Department of Cardiology, The Second Affiliated Hospital of Zhengzhou University, Zhengzhou 450014, China.
Department of Cardiology, The Second Affiliated Hospital of Zhengzhou University, Zhengzhou 450014, China.
Stem Cell Res. 2023 Mar;67:103029. doi: 10.1016/j.scr.2023.103029. Epub 2023 Jan 16.
Protein phosphatase 2A (PPP2CA) is one of the four main Ser/Thr phosphatase enzymes, which involved in the negative control of cell growth and division. PPP2CA is the main protein phosphatase in the heart, which regulates Ca (2+) through a series of ion channels and transporters. In this study, we generated a PPP2CA homozygous knockout human embryonic stem cell line WAe009-A-25 based on the transient expression CRISPR/Cas9 system to investigate functional effect of PP1 deficiency. This cell line has multidirectional differentiation potential, normal karyotypic and trilineage differentiation potential in vivo.
