通过 CRISPR/Cas9 生成 PPP1CA 基因敲除的人多能干细胞系。
Generation of a PPP1CA knockout human pluripotent stem cell line via CRISPR/Cas9.
机构信息
Department of Emergency, Zhengzhou Central Hospital Affiliated to Zhengzhou University, Zhengzhou 450007, China.
Henan Institute of Metrology, No. 10 Baifo Road, Guancheng District, Zhengzhou 450008, China.
出版信息
Stem Cell Res. 2023 Jun;69:103077. doi: 10.1016/j.scr.2023.103077. Epub 2023 Mar 21.
The Protein phosphatase 1 catalytic subunit alpha (PPP1CA) is a alpha subunit of the PP1 complex, which known to be involved in the regulation of a variety of cellular processes, such as cell division, glycogen metabolism, muscle contractility, protein synthesis, and HIV-1 viral transcription. Increased PP1 activity has been observed in the end stage of heart failure. Here, a PPP1CA knockout human embryonic stem cell line, WAe009-A-B, was generated using the CRISPR/Cas9 system to further study the function of PPP1CA. The cell line confirmed with pluripotency, normal karyotype and differentiation potential.
蛋白磷酸酶 1 催化亚基 alpha（PPP1CA）是 PP1 复合物的 alpha 亚基，已知其参与调节多种细胞过程，如细胞分裂、糖原代谢、肌肉收缩、蛋白质合成和 HIV-1 病毒转录。在心力衰竭的终末期观察到 PP1 活性增加。在这里，使用 CRISPR/Cas9 系统生成了 PPP1CA 敲除的人胚胎干细胞系 WAe009-A-B，以进一步研究 PPP1CA 的功能。该细胞系经多能性、正常核型和分化潜能证实。
Zotero 插件