Department of Chemistry and Sarafan ChEM-H, Stanford University, Stanford, CA, United States.
Department of Urology, Stanford University School of Medicine, Stanford, CA, United States.
Adv Cancer Res. 2023;157:83-121. doi: 10.1016/bs.acr.2022.09.001. Epub 2022 Oct 8.
Mucin-domain glycoproteins are highly O-glycosylated cell surface and secreted proteins that serve as both biochemical and biophysical modulators. Aberrant expression and glycosylation of mucins are known hallmarks in numerous malignancies, yet mucin-domain glycoproteins remain enigmatic in the broad landscape of cancer glycobiology. Here we review the multifaceted roles of mucins in cancer through the lens of the analytical and biochemical methods used to study them. We also describe a collection of emerging tools that are specifically equipped to characterize mucin-domain glycoproteins in complex biological backgrounds. These approaches are poised to further elucidate how mucin biology can be understood and subsequently targeted for the next generation of cancer therapeutics.
黏蛋白型糖蛋白是高度 O-糖基化的细胞表面和分泌蛋白，可作为生化和生物物理调节剂。黏蛋白的异常表达和糖基化是许多恶性肿瘤的显著特征，但在癌症糖生物学的广阔领域中，黏蛋白型糖蛋白仍然是一个谜。本文通过研究黏蛋白的分析和生化方法，从多个角度综述了黏蛋白在癌症中的作用。我们还描述了一系列新兴工具，这些工具专门用于在复杂的生物学背景下对黏蛋白结构域糖蛋白进行特征分析。这些方法有望进一步阐明如何理解黏蛋白生物学，并为下一代癌症治疗方法提供靶标。
