College of Food Science and Technology, Nanjing Agricultural University, Nanjing, Jiangsu 210095, China.
Institute of Wolfberry Engineering Technology, Ningxia Academy of Agriculture and Forestry Sciences, Yinchuan, Ningxia 750002, China.
J Agric Food Chem. 2023 Feb 15;71(6):2864-2882. doi: 10.1021/acs.jafc.2c06713. Epub 2023 Feb 1.
In the present study, we found that anthocyanins from Murray (ACN) potently ameliorated a high-fructose diet (HFrD)-induced neuroinflammation in mice. ACN improved the integrity of the intestinal barrier and suppressed the toll-like receptor 4 (TLR4) signaling pathway to ameliorate the neuroinflammation, which was verified by 4 mice. Furthermore, ACN could modulate the HFrD-induced dysbiosis of gut microbiota. The fecal microbiota transplantation from ACN-induced mice was sufficient to attenuate the neuroinflammation, while the amelioration of neuroinflammation by ACN was blocked upon gut microbiota depletion. In addition, ACN-induced increment of the relative abundance of might be responsible for the alleviation of the neuroinflammation, which was further confirmed in the promoting effect of ACN on the growth of in vitro. Overall, these results provided the evidence of a comprehensive cross-talk mechanism between ACN and neuroinflammation in HFrD-fed mice, which was mediated by reducing gut microbiota dysbiosis and maintaining the intestinal barrier integrity.
在本研究中，我们发现来自 Murray（ACN）的花色苷可有效改善高果糖饮食（HFrD）诱导的小鼠神经炎症。ACN 可改善肠道屏障的完整性，并抑制 Toll 样受体 4（TLR4）信号通路，从而改善神经炎症，这在 4 只小鼠中得到了验证。此外，ACN 可调节 HFrD 诱导的肠道微生物群失调。来自 ACN 诱导的小鼠的粪便微生物群移植足以减轻神经炎症，而在肠道微生物群耗竭后，ACN 对神经炎症的改善作用被阻断。此外，ACN 诱导的相对丰度的增加可能是减轻神经炎症的原因，这在 ACN 促进体外生长的作用中得到了进一步证实。总的来说，这些结果提供了证据，表明 ACN 与 HFrD 喂养小鼠的神经炎症之间存在全面的相互作用机制，该机制通过减少肠道微生物群失调和维持肠道屏障完整性来介导。
