Department of Animal Production and Preventive Veterinary Medicine, School of Veterinary Medicine and Animal Sciences, São Paulo State University-UNESP, 18618-681 Botucatu, SP, Brazil.
Department of Animal Biology, Institute of Biology, University of Campinas-UNICAMP, Campinas, 13083-862 SP, Brazil.
J Appl Microbiol. 2023 Mar 1;134(3). doi: 10.1093/jambio/lxad020.
We aimed to investigate the prevalence of rotavirus and coronavirus in dipterans that commonly inhabit the environment of dairy farms.
We collected 217 insect specimens from nine dairy farms, which were examined through hemi-nested RT-PCR followed by Sanger sequencing in search of VP1 and N genes for rotavirus and bovine coronavirus-BCoV, respectively. With a predominance of Muscidae (152/217 = 70%) 11 families of Diptera were identified. Rotavirus A (RVA) and betacoronavirus (BCoV) were detected in 14.7% (32/217) and 4.6% (10/217) of the dipterans, respectively. Sequencing of the amplicons was possible for 11.5% (25/217) of RVA and 0.5% (1/217) of BCoV, confirming the presence of these pathogens.
Our findings highlight the role of dipterans as carriers of RVA and BCoV of great relevance for public and animal health.
我们旨在调查常见于奶牛场环境中的双翅目昆虫中轮状病毒和冠状病毒的流行情况。
我们从 9 个奶牛场收集了 217 个昆虫标本，通过半巢式 RT-PCR 进行检测，并对 VP1 和 N 基因进行 Sanger 测序，分别寻找轮状病毒和牛冠状病毒-BCoV。在 217 个标本中，优势为蝇科（152/217=70%），共鉴定出 11 个科的双翅目昆虫。轮状病毒 A（RVA）和β冠状病毒（BCoV）分别在 14.7%（32/217）和 4.6%（10/217）的双翅目昆虫中被检测到。11.5%（25/217）的 RVA 和 0.5%（1/217）的 BCoV 的扩增子测序是可能的，证实了这些病原体的存在。
我们的研究结果强调了双翅目昆虫作为轮状病毒和牛冠状病毒的携带者的作用，这些病毒对公共卫生和动物健康具有重要意义。
