College of Pharmacy, Hunan University of Chinese Medicine, Changsha, China.
Key Laboratory of Germplasm Resources and Standardized Planting of Hunan Large-scale Genuine Medicinal Materials, Changsha, China.
Phytochem Anal. 2024 Jul;35(5):1221-1248. doi: 10.1002/pca.3355. Epub 2024 Apr 19.
The active constituents in Aurantii Fructus sourced from different regions within Hunan Province exhibit variations, with certain samples demonstrating substandard quality.
The aim of this study is to conduct a comparative analysis of the chemical composition and quality of Aurantii Fructus from various sources, establish a robust methodology for quality evaluation, and determine the optimal harvesting period.
The components of Aurantii Fructus were qualitatively analyzed using a non-targeted metabolomics approach. Multivariate statistical analyses were conducted to identify potential markers, enabling qualitative and quantitative evaluation of the quality and optimal harvest period of Aurantii Fructus.
Overall, 155 compounds were identified in Aurantii Fructus, with Huangpi exhibiting the highest number of components. Eleven potential markers were selected to assess the quality of Aurantii Fructus. The average content of Huangpi was the highest, indicating a high level of similarity. The samples' overall scores were ordered as follows: Huangpi > Xiangcheng > Choucheng > Daidai. Anren and Changde's Huangpi exhibited high contents, being rich in chemical components, resulting in favorable scores. Similarly, Changde's Xiangcheng displayed significant medicinal value. As the harvest time was delayed, there was an increase in fruit size, accompanied by thinner peels and a continuous decrease in the contents of potential markers. The best harvest period of Aurantii Fructus was within 1 week before and after the Lesser Heat.
The present study establishes a precise and efficient method for evaluating the quality of Aurantii Fructus, thereby providing more comprehensive insights into its composition. This research lays the foundation for subsequent development and utilization of Aurantii Fructus.
湖南省不同地区产的枳实，其活性成分存在差异，部分样品质量不达标。
本研究旨在对不同来源枳实的化学成分和质量进行比较分析，建立一种稳健的质量评价方法，并确定最佳采收期。
采用非靶向代谢组学方法对枳实成分进行定性分析。运用多变量统计分析方法，筛选潜在标志物，实现对枳实质量和最佳采收期的定性和定量评价。
共鉴定出枳实中的 155 种成分，黄皮中成分种类最多。筛选出 11 个潜在标志物用于评估枳实的质量。黄皮的平均含量最高，表明相似度较高。各样本的综合评分依次为：黄皮>湘橙>稠皮>代代。安仁和常德的黄皮含量较高，化学成分丰富，得分较高。同样，常德的湘橙具有显著的药用价值。随着采收时间的推迟，果实逐渐增大，果皮变薄，潜在标志物的含量持续下降。枳实的最佳采收期在小暑前后 1 周内。
本研究建立了一种准确、高效的枳实质量评价方法，为其成分提供了更全面的认识。本研究为枳实的后续开发利用奠定了基础。
