Department of Biomedical, Surgical and Dental Sciences, Università degli Studi di Milano, Milan, Italy.
J Periodontal Res. 2023 Apr;58(2):422-432. doi: 10.1111/jre.13103. Epub 2023 Feb 2.
The aim of the present study was to assess if the endocannabinoid system is involved differently in patients with recurrent and non-recurrent periodontal disease and if in sites that have a predisposition for reactivation, levels of anandamide (AEA) change after periodontal therapy.
Periodontal disease (PD) may be due to a dysregulation of the endocannabinoid system.
Periodontal patients were recruited, treated for PD and monitored. Gingival samples from these patients with recurrent (n = 10) and non-recurrent (n = 10) periodontal disease were harvested before and after treatment and compared to those of periodontally healthy (n = 10) subjects. Levels of CB1 and CB2, AEA and CBs receptor activation were assessed in healthy and inflamed samples using immunohistochemistry, chromatography and autoradiography. In healed sites, AEA levels were also assessed.
The number of CBs in inflamed sites of recurrent patients was significantly higher than in those with non-recurrent disease and also higher than those in healthy subjects. Inflamed sites of recurrent patients had significantly lower CBs receptor activation than those of healthy subjects. Levels of AEA in inflamed sites of non-recurrent patients were significantly higher than those found both in inflamed recurrent sites and in healthy sites. Otherwise, the amount of AEA in healthy subjects and recurrent inflamed sites was similar. After periodontal therapy, levels of AEA were significantly lower in both periodontal groups. In recurrent sites, they resulted significantly lower than in non-recurrent and even in healthy subjects.
The endocannabinoid system seems involved differently in subjects with recurrent and non-recurrent periodontal disease.
本研究旨在评估内源性大麻素系统在复发性和非复发性牙周病患者中是否存在不同的参与，以及在易复发部位，经牙周治疗后，大麻素（AEA）的水平是否会发生变化。
牙周病（PD）可能是由于内源性大麻素系统的失调。
招募牙周病患者，对其进行 PD 治疗并进行监测。从这些复发性（n=10）和非复发性（n=10）牙周病患者以及牙周健康患者（n=10）的牙龈样本中采集治疗前后的样本，并进行比较。使用免疫组织化学、色谱和放射自显影法评估 CB1 和 CB2、AEA 和 CB 受体激活在健康和炎症样本中的水平。在愈合部位，还评估了 AEA 的水平。
复发性患者炎症部位的 CBs 数量明显高于非复发性疾病患者，也高于健康受试者。复发性患者炎症部位的 CB 受体激活明显低于健康受试者。非复发性患者炎症部位的 AEA 水平明显高于炎症部位的复发性患者和健康受试者。此外，健康受试者和复发性炎症部位的 AEA 含量相似。牙周治疗后，两组牙周炎患者的 AEA 水平均显著降低。在复发性部位，其水平明显低于非复发性部位，甚至低于健康部位。
内源性大麻素系统在复发性和非复发性牙周病患者中的参与似乎不同。
