Hedstrom O R, Sonn R J, Lassen E D, Hultgren B D, Crisman R O, Smith B B, Snyder S P
Veterinary Diagnostic Laboratory, Veterinary Teaching Hospital, Corvallis, OR.
Vet Pathol. 1987 Sep;24(5):419-26. doi: 10.1177/030098588702400509.
Campylobacter jejuni was inoculated intravenously into pregnant ewes on gestation days 114 and 123 to reproduce ovine abortion. All ewes aborted 7-12 days post-inoculation. High numbers of C. jejuni were isolated from ewe tissues (caruncle, bile, cecal feces), fetal tissues, and placenta. C. jejuni colonies were identified in caruncles and placenta by light microscopy and immunoperoxidase techniques. Histologically, inoculated ewes had a severe purulent endometritis with vasculitis. Placentas from inoculated ewes and field cases showed necrosis and purulent inflammation; however, placentas from inoculated ewes had large numbers of bacterial colonies compared to few bacteria found in field cases. Histologically, only one fetus from the inoculated ewes showed lesions (purulent bronchopneumonia), whereas all fetuses from field cases had a distinct bronchopneumonia, and one fetus showed multifocal hepatic necrosis. These results suggest that C. jejuni (serotypes Penner 1 and Lior 2) is an important abortifacient organism for sheep.
在妊娠第114天和123天，将空肠弯曲菌静脉注射到怀孕母羊体内以引发绵羊流产。所有母羊在接种后7 - 12天流产。从母羊组织（肉阜、胆汁、盲肠粪便）、胎儿组织和胎盘中分离出大量空肠弯曲菌。通过光学显微镜和免疫过氧化物酶技术在肉阜和胎盘中鉴定出空肠弯曲菌菌落。组织学上，接种母羊患有严重的脓性子宫内膜炎并伴有血管炎。接种母羊和现场病例的胎盘显示坏死和脓性炎症；然而，与现场病例中发现的少量细菌相比，接种母羊的胎盘有大量细菌菌落。组织学上，接种母羊中只有一只胎儿出现病变（脓性支气管肺炎），而现场病例中的所有胎儿都有明显的支气管肺炎，并且一只胎儿出现多灶性肝坏死。这些结果表明空肠弯曲菌（彭纳血清型1和利奥尔血清型2）是绵羊重要的流产病原体。
