Department of Veterinary Clinical Sciences, Center for Veterinary Health Sciences, Oklahoma State University, Stillwater, Oklahoma, United States of America.
Department of Animal Science, Oklahoma State University, Stillwater, Oklahoma, United States Of America.
PLoS One. 2019 Jan 7;14(1):e0210157. doi: 10.1371/journal.pone.0210157. eCollection 2019.
The objective of this study was to characterize the normal microbiome of healthy canine vagina and endometrium and to determine the effect of the stage of estrous, on the resident microbiome. Cranial vaginal swabs and uterine biopsy samples were collected from twenty-five bitches in five different stages of estrous at elective ovariohysterectomy (OVH). Over 4 million reads of the V4 region of 16S rDNA gene were obtained and used for further analyses. A total of 317 genera belonging to 24 known phyla were identified. The endometrium was higher in bacterial diversity while the vagina was higher in richness. Proteobacteria, Bacteroidetes and Firmicutes were the most abundant phyla observed across all samples. Hydrotalea, Ralstonia, and Fusobacterium accounted for nearly 60% of the OTUs identified in the vagina while organisms identified in the endometrium were more evenly distributed. Pseudomonas, Staphylococcus, and Corynebacterium were the prominent genera in the endometrium. The microbiome of the endometrium was distinctly different from that of the vagina. There was large animal-to-animal variation. Other than the vaginal microbiome of bitches in estrus (i.e. in heat), there were no distinct clustering of the organisms based on the stage of estrous. These findings establish the presence of a resident microbiome of the endometrium throughout all stages of estrous cycle.
本研究旨在描述健康犬阴道和子宫内膜的正常微生物组，并确定发情周期各阶段对常驻微生物组的影响。在选择性卵巢子宫切除术（OVH）时，从 25 只发情母犬的五个不同发情阶段采集了阴道顶端拭子和子宫活检样本。获得了超过 400 万个 16S rDNA 基因 V4 区的读取序列，并用于进一步分析。共鉴定出 24 个已知门的 317 个属。与所有样本相比，子宫内膜的细菌多样性更高，而阴道的丰富度更高。在所有样本中，变形菌门、拟杆菌门和厚壁菌门是最丰富的门。在阴道中，水栖杆菌属、罗尔斯顿氏菌属和梭杆菌属占鉴定出的 OTU 的近 60%，而在子宫内膜中鉴定出的微生物则分布更为均匀。假单胞菌属、葡萄球菌属和棒状杆菌属是子宫内膜中的主要属。子宫内膜的微生物组与阴道的微生物组明显不同。个体间存在较大差异。除发情母犬的阴道微生物组（即发情）外，根据发情阶段，没有明显的聚类现象。这些发现确立了发情周期各个阶段都存在子宫内膜常驻微生物组。
