Khdary Nezar H, Alangari Abdulaziz A, Katubi Khadijah M, Alanazi Mohammad, Alhassan Ahmed, Alzahrani Sami D, Khan Zahid, Alanazi Ibrahim O
Institute of Materials Science, King Abdulaziz City for Science and Technology, Riyadh, Kingdom of Saudi Arabia.
College of Medicine, King Saud University, Riyadh, Kingdom of Saudi Arabia.
Cancer Manag Res. 2023 Jan 27;15:87-98. doi: 10.2147/CMAR.S391546. eCollection 2023.
Herbs are excellent sources of medicinal substances, and their curative abilities have been recognized to treat many ailments and are used for example as antioxidants, analgesics, anti-inflammatories, antipyretics, and many other medicinal uses. The properties of natural compounds and their health effects have been studied extensively, especially those that originate from plant sources such as ginger. The ginger plant contains many chemical compounds, such as 6-gingerol, which is characterized by containing active groups such as carbonyl and hydroxide, which can be attached to metal molecules. This is what was done in this study, where the formation of complexes with a group of metals was studied and their effect on cancer cells was investigated. These complexes will open new horizons for further study of medicinal uses.
The synthesis of gingerol-metal complexes was carried out by conjugating gingerol molecules with Ag, Au, Cd, Co, Cu, Ni, and Zn metal ions. The extracted gingerol was transferred to culture tubes and deionized water-DMSO were added followed by sonication. The tubes were incubated at 90°C for two days as well as the control sample. The samples were then filtered and the complex solutions were transferred into new tubes for further studies. Different characterization techniques such as FT-IR, UV-vis spectroscopy, FESEM, and EDX are used to confirm the formation of the complexes. The in vitro of the complexes was tested by the 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) assay against the human colorectal cancer cell lines HCT116 and HT29 which exhibited strong cytotoxicity.
The gingerol-metal complexes showed an enhancement as an anticancer agent compared to the control. The in vitro anticancer activity showed that the Ag-gingerol complex showed the most activity among the other complexes.
Gingerol-metal complexes can inhibit cancer cells, noting that the potency of the complex depends on the type of metal used.
草药是药用物质的优质来源，其治疗能力已被认可可用于治疗多种疾病，例如用作抗氧化剂、止痛剂、抗炎药、退烧药以及许多其他药用用途。天然化合物的特性及其对健康的影响已得到广泛研究，尤其是那些源自植物来源（如生姜）的化合物。生姜植物含有许多化合物，如6-姜酚，其特征在于含有羰基和羟基等活性基团，这些基团可与金属分子结合。本研究正是如此，研究了与一组金属形成络合物及其对癌细胞的影响。这些络合物将为药用用途的进一步研究开辟新的视野。
通过将姜酚分子与银、金、镉、钴、铜、镍和锌金属离子共轭来进行姜酚-金属络合物的合成。将提取的姜酚转移到培养管中，加入去离子水-二甲基亚砜，然后进行超声处理。将试管与对照样品一起在90°C下孵育两天。然后对样品进行过滤，并将络合物溶液转移到新的试管中进行进一步研究。使用不同的表征技术，如傅里叶变换红外光谱（FT-IR）、紫外可见光谱、场发射扫描电子显微镜（FESEM）和能谱分析（EDX）来确认络合物的形成。通过3-(4,5-二甲基噻唑-2-基)-5-(3-羧甲氧基苯基)-2-(4-磺基苯基)-2H-四唑鎓（MTS）测定法对络合物进行体外测试，针对人结肠癌细胞系HCT116和HT29，其表现出很强的细胞毒性。
与对照相比，姜酚-金属络合物作为抗癌剂表现出增强作用。体外抗癌活性表明，银-姜酚络合物在其他络合物中表现出最强活性。
姜酚-金属络合物可以抑制癌细胞，注意到络合物的效力取决于所使用的金属类型。
