Department of Biotechnology, Quaid-i-Azam University, Islamabad 45320, Pakistan; Department of Biotechnology, University of Malakand, Chakdara, KP, Pakistan.
Department of Biotechnology, Quaid-i-Azam University, Islamabad 45320, Pakistan.
J Photochem Photobiol B. 2018 Aug;185:153-160. doi: 10.1016/j.jphotobiol.2018.06.002. Epub 2018 Jun 9.
Manipulation in the light regimes combined with the effects of plant growth regulators (PGRs) and elicitors through plant cell culture technology is a promising strategy for enhancing the yield of medicinally important secondary metabolites. In this study, the effects of interplay between PGRs, elicitors and light regimes on cell cultures of F. indica have been investigated. The results showed that callus cultures resulted in maximum biomass formation (13.2 g/L) when incubated on solid MS (Murashige and Skoog) medium containing 1.0 mg/L BA under continuous light for 4 weeks. Among the other PGRs, compared with the auxins such as 2,4-D, and IBA, TDZ resulted in higher biomass accumulation (12.1 g/L). Elicitors (Me-J and PAA) resulted in a lower growth response, when compared with cytokinins and a higher response than auxins under all the light regimes on solid MS media. However, in liquid media, no significant increase in biomass was observed in response to the combined effects of PGRs and photoperiod regimes. Further, the highest phenolic content (TPC = 6.8 mg) and flavonoid content (TFC = 5.2 mg) were detected in the dark-grown cell cultures raised in vitro at 0.5 mg/L Me-J. The highest antioxidant activity (88%) was recorded in the dark-grown cell cultures harvested from LOG phase of the growth cycle supplemented with 0.5 mg/L Me-J. Furthermore, BA resulted in considerable flavonoids production (TFC = 4.7 mg) in the cell cultures grown under continuous light. However, overall dark treatment and elicitation with Me-J resulted in the optimal metabolic response in terms of secondary metabolites accumulation in cell suspension cultures of F. indica.
通过植物细胞培养技术，在光照条件下操纵与植物生长调节剂（PGRs）和诱导子的相互作用，是提高药用次生代谢物产量的一种很有前途的策略。本研究探讨了 PGRs、诱导子和光照条件对 F. indica 细胞培养的相互作用的影响。结果表明，在连续光照下，固体 MS（Murashige 和 Skoog）培养基中添加 1.0mg/L BA 时，愈伤组织培养物的生物量形成最大（13.2g/L）。在其他 PGRs 中，与 2,4-D 和 IBA 等生长素相比，TDZ 导致更高的生物量积累（12.1g/L）。与细胞分裂素相比，诱导子（Me-J 和 PAA）在固体 MS 培养基的所有光照条件下的生长反应较低，但比生长素的反应更高。然而，在液体培养基中，没有观察到 PGRs 和光周期条件的组合效应导致生物量显著增加。此外，在黑暗中培养的细胞中检测到最高的酚含量（TPC=6.8mg）和类黄酮含量（TFC=5.2mg）在 0.5mg/L Me-J 下体外生长。在补充 0.5mg/L Me-J 的生长周期 LOG 阶段收获的黑暗培养细胞中记录到最高的抗氧化活性（88%）。此外，BA 在连续光照下培养的细胞中产生相当数量的类黄酮（TFC=4.7mg）。然而，总体上黑暗处理和用 Me-J 诱导导致 F. indica 细胞悬浮培养物中次生代谢物积累的最佳代谢反应。
