Yang Shang, Tang Rong, Xie Cheng-Jie, Han Shuang, Rensing Christopher, Liu Guo-Hong, Zhou Shun-Gui
Fujian Provincial Key Laboratory of Soil Environmental Health and Regulation, College of Resources and Environment, Fujian Agriculture and Forestry University, Fuzhou City, 350002, Fujian Province, People's Republic of China.
Agricultural Bio-Resources Research Institute, Fujian Academy of Agricultural Sciences, Fuzhou City, 350003, Fujian Province, People's Republic of China.
Arch Microbiol. 2023 Feb 3;205(3):80. doi: 10.1007/s00203-023-03412-3.
A strictly anaerobic sulfate-reducing strain, designated SG60, was isolated from paddy soil collected in Fujian Province, China. Growth of strain SG60 was observed at 20-37 °C, pH 5.5-10.0 and 0-0.7% (w/v) NaCl. Strain SG60 showed the highest 16S rRNA sequence similarities to the type strains of Fundidesulfovibrio magnetotacticus FSS-1 (97.2%) and Fundidesulfovibrio putealis DSM 16056 (96.4%). Phylogenetic trees based on the16S rRNA sequence and genome-based phylogenomic tree constructed using 120 core genes showed that strain SG60 clustered with members of the genus Fundidesulfovibrio. The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values between strain SG60 and the most closely related type strain F. magnetotacticus were 78.2% and 21.6%, respectively. Strain SG60 contained MK-7 as the main respiratory quinone and anteiso-C, anteiso-C ω9c, iso-C and iso-C H as the major fatty acids. Strain SG60 produced desulfoviridin and possessed genes (nifHDK) encoding functions involved in nitrogen fixation. The genomic DNA G + C content was 65.5%. Based on the observed physiological properties, chemotaxonomic characteristics and ANI and dDDH values, strain SG60 represents a novel species of the genus Fundidesulfovibrio, for which the name Fundidesulfovibrio soli sp. nov. is proposed. The type strain is SG60 (= GDMCC 1.3310 = JCM 35676).
从中国福建省采集的稻田土壤中分离出一株严格厌氧的硫酸盐还原菌株，命名为SG60。在20-37°C、pH 5.5-10.0和0-0.7%（w/v）NaCl条件下观察到菌株SG60的生长。菌株SG60与趋磁基金脱硫弧菌FSS-1（97.2%）和腐殖基金脱硫弧菌DSM 16056（96.4%）的模式菌株显示出最高的16S rRNA序列相似性。基于16S rRNA序列构建的系统发育树和使用120个核心基因构建的基于基因组的系统发育基因组树表明，菌株SG60与基金脱硫弧菌属的成员聚类。菌株SG60与最密切相关的模式菌株趋磁基金脱硫弧菌之间的平均核苷酸同一性（ANI）和数字DNA-DNA杂交（dDDH）值分别为78.2%和21.6%。菌株SG60含有MK-7作为主要呼吸醌，anteiso-C、anteiso-C ω9c、iso-C和iso-C H作为主要脂肪酸。菌株SG60产生脱硫绿素，并拥有编码参与固氮功能的基因（nifHDK）。基因组DNA G+C含量为65.5%。基于观察到的生理特性、化学分类特征以及ANI和dDDH值，菌株SG60代表基金脱硫弧菌属的一个新物种，为此提出名称腐殖基金脱硫弧菌新种（Fundidesulfovibrio soli sp. nov.）。模式菌株为SG60（=GDMCC 1.3310=JCM 35676）。
