Fujian Provincial Key Laboratory of Soil Environmental Health and Regulation, College of Resources and Environment, Fujian Agriculture and Forestry University, Fuzhou City, Fujian Province, 350002, People's Republic of China.
Guangdong Key Laboratory of Environmental Catalysis and Health Risk Control, School of Environmental Science and Engineering, Institute of Environmental Health and Pollution Control, Guangdong University of Technology, Guangzhou City, Guangdong Province, 510006, People's Republic of China.
Antonie Van Leeuwenhoek. 2024 May 17;117(1):79. doi: 10.1007/s10482-024-01978-6.
A nitrogen-fixing strain designated SG130 was isolated from paddy soil in Fujian Province, China. Strain SG130 was Gram-staining-negative, rod-shaped, and strictly anaerobic. Strain SG130 showed the highest 16S rRNA gene sequence similarities with the type strains Dendrosporobacter quercicolus DSM 1736 (91.7%), Anaeroarcus burkinensis DSM 6283 (91.0%) and Anaerospora hongkongensis HKU 15 (90.9%). Furthermore, the phylogenetic and phylogenomic analysis also suggested strain SG130 clustered with members of the family Sporomusaceae and was distinguished from other genera within this family. Growth of strain SG130 was observed at 25-45 °C (optimum 30 °C), pH 6.0-9.5 (optimum 7.0) and 0-1% (w/v) NaCl (optimum 0.1%). The quinones were Q-8 and Q-9. The polar lipids were phosphatidylserine (PS), phosphatidylethanolamine (PE), glycolipid (GL), phospholipid (PL) and an unidentified lipid (UL). The major fatty acids (> 10%) were iso-C 3OH (26.6%), iso-C (15.6%) and iso-C F (11.4%). The genomic DNA G + C content was 50.7%. The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values between strain SG130 and the most closely related type strain D. quercicolus DSM 1736 (ANI 68.0% and dDDH 20.3%) were both below the cut-off level for species delineation. The average amino acid identity (AAI) between strain SG130 and the most closely related type strain D. quercicolus DSM 1736 was 63.2%, which was below the cut-off value for bacterial genus delineation (65%). Strain SG130 possessed core genes (nifHDK) involved in nitrogen fixation, and nitrogenase activity (106.38 μmol CH g protein h) was examined using the acetylene reduction assay. Based on the above results, strain SG130 is confirmed to represent a novel genus of the family Sporomusaceae, for which the name Azotosporobacter soli gen. nov., sp. nov. is proposed. The type strain is SG130 (= GDMCC 1.3312 = JCM 35641).
一株固氮菌株 SG130 从中国福建省稻田土壤中分离得到。菌株 SG130 革兰氏染色阴性，呈杆状，严格厌氧。菌株 SG130 的 16S rRNA 基因序列与模式菌株 Dendrosporobacter quercicolus DSM 1736（91.7%）、Anaeroarcus burkinensis DSM 6283（91.0%）和 Anaerospora hongkongensis HKU 15（90.9%）的相似度最高。此外，系统发育和系统基因组分析还表明，菌株 SG130 与 Sporomusaceae 家族的成员聚类，并与该家族内的其他属区分开来。菌株 SG130 的生长温度为 25-45°C（最佳温度 30°C），pH 值为 6.0-9.5（最佳 pH 值为 7.0）和 0-1%（w/v）NaCl（最佳 0.1%）。其醌类物质为 Q-8 和 Q-9。极性脂类为磷脂酰丝氨酸（PS）、磷脂酰乙醇胺（PE）、糖脂（GL）、磷脂（PL）和一种未知脂类（UL）。主要脂肪酸（>10%）为 iso-C 3OH（26.6%）、iso-C（15.6%）和 iso-C F（11.4%）。基因组 DNA G+C 含量为 50.7%。菌株 SG130 与最密切相关的模式菌株 D. quercicolus DSM 1736 的平均核苷酸同一性（ANI）和数字 DNA-DNA 杂交（dDDH）值分别为 68.0%和 20.3%，均低于种系划分的临界值。菌株 SG130 与最密切相关的模式菌株 D. quercicolus DSM 1736 的平均氨基酸同一性（AAI）为 63.2%，低于细菌属系划分的临界值（65%）。菌株 SG130 拥有参与固氮的核心基因（nifHDK），并通过乙炔还原测定法检测到其固氮酶活性（106.38 μmol CH g protein h）。基于上述结果，确认菌株 SG130 代表 Sporomusaceae 家族的一个新属，命名为 Azotosporobacter soli gen. nov.，sp. nov.。其模式菌株为 SG130（=GDMCC 1.3312=JCM 35641）。
