Yang Shang, Tang Rong, Han Shuang, Xie Cheng-Jie, Liu Guo-Hong, Zhou Shun-Gui
Fujian Provincial Key Laboratory of Soil Environmental Health and Regulation, College of Resources and Environment, Fujian Agriculture and Forestry University, Fuzhou City, 350002, PR China.
Agricultural Bio-resources Research Institute, Fujian Academy of Agricultural Sciences, Fuzhou City, 350003, PR China.
Int J Syst Evol Microbiol. 2023 May;73(5). doi: 10.1099/ijsem.0.005880.
A strictly anaerobic sulfate-reducing strain, designated SG127, was isolated from paddy soil. SG127 showed the highest 16S rRNA gene sequence similarity to the type strain of (98.2 %). A phylogenetic tree based on 16S rRNA gene sequences indicated that SG127 clustered with members of the genus . Growth of SG127 was observed at 20-37 °C (optimum, 30 °C), pH 5.5-9.0 (optimum, 7.0-8.0) and with 0-0.2 % (w/v) NaCl (optimally without NaCl). SG127 contained MK-7 as the only menaquinone and anteiso-C, anteiso-Cω9, C, iso-C iso-C, iso-C iso-CH, iso-CH and summed feature nine as the major fatty acids. The genomic DNA G+C content of SG127 was 64.6 %. The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values between SG127 and the closely related were 78.5% and 23.2 %, respectively, which were lower than the cut-off values (ANI 95-96% and dDDH 70 %) for prokaryotic species delineation. SG127 had desulfoviridin, possessed nitrogen fixation genes () and actively fixed nitrogen according to the acetylene reduction assay. On the basis of these results, strain SG127 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is SG127 (= GDMCC 1.3137 = JCM 35589).
从稻田土壤中分离出一株严格厌氧的硫酸盐还原菌株，命名为SG127。SG127的16S rRNA基因序列与[某菌株]模式菌株的相似度最高（98.2%）。基于16S rRNA基因序列构建的系统发育树表明，SG127与[某属]的成员聚类在一起。在20-37°C（最适温度为30°C）、pH 5.5-9.0（最适pH为7.0-8.0）以及0-0.2%（w/v）NaCl（最适无NaCl）条件下可观察到SG127的生长。SG127含有MK-7作为唯一的甲基萘醌，以及anteiso-C、anteiso-Cω9、C、iso-C、iso-C、iso-C、iso-C、iso-CH和总和特征9作为主要脂肪酸。SG127的基因组DNA G+C含量为64.6%。SG127与密切相关的[某菌株]之间的平均核苷酸同一性（ANI）和数字DNA-DNA杂交（dDDH）值分别为78.5%和23.2%，低于原核生物物种划分的临界值（ANI 95-96%和dDDH 70%）。SG127具有脱硫绿素，拥有固氮基因（[具体基因名称]），并且根据乙炔还原试验可积极固氮。基于这些结果，菌株SG127代表[某属]的一个新物种，为此提出新名称[新物种名称] sp. nov.。模式菌株为SG127（= GDMCC 1.3137 = JCM 35589）。
