新格雷霉素，一种三硫桥键联的安卡环类抗生素，在表达硫安卡环生物合成基因簇的 J1074 中产生。
Neogrisemycin, a Trisulfide-Bridged Angucycline, Produced upon Expressing the Thioangucycline Biosynthetic Gene Cluster in J1074.
机构信息
Skaggs Graduate School of Chemical and Biological Sciences, Scripps Research, Jupiter, Florida 33458, United States.
出版信息
Org Lett. 2023 Feb 17;25(6):961-965. doi: 10.1021/acs.orglett.2c04303. Epub 2023 Feb 3.
Abstract
Neogrisemycin () was isolated from recombinant J1074 strain SB4061 expressing an engineered thioangucycline (TAC) biosynthetic gene cluster (BGC). The structure and absolute configuration of were established by a combination of mass spectrometry, nuclear magnetic resonance, and single-crystal X-ray diffraction analyses. Like the TACs, was also proposed to derive non-enzymatically from the common epoxide (), the nascent product encoded by the BGC, mediated by endogenous hydrogen trisulfide.
摘要
新格雷西霉素（）是从表达工程化硫杂蒽环（TAC）生物合成基因簇（BGC）的重组 J1074 菌株 SB4061 中分离得到的。通过质谱、核磁共振和单晶 X 射线衍射分析的组合，确定了的结构和绝对构型。与 TAC 类似，也被认为是由共同的环氧化物（）非酶促衍生而来的，该环氧化物是 BGC 编码的初生产物，由内源性氢三硫化物介导。
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